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| 关木通HPLC-DAD-ESI/MS指纹图谱研究 | |
| 引用本文： | 樊夏雷,丁一冰,冯有龙,尚姝,刘文英. 关木通HPLC-DAD-ESI/MS指纹图谱研究[J]. 中草药, 2008, 39(12): 1883-1887 |
| 作者姓名： | 樊夏雷 丁一冰 冯有龙 尚姝 刘文英 |
| 作者单位： | 1. 中国药科大学,江苏,南京,210009;江苏省药品检验所,江苏,南京,210008 2. 南京大学医学院,江苏,南京,210008 3. 江苏省药品检验所,江苏,南京,210008 4. 中国药科大学,江苏,南京,210009 |
| 摘 要： | 目的建立关木通HPLC-DAD-ESI/MS指纹图谱分析方法,可用于关木通质量的评价,为进一步开展关木通的肾毒性代谢研究提供药材质量依据。方法用75%甲醇对不同产地的关木通样品进行提取,经HPLC-DAD-ESI/MS联用技术分析,建立指纹图谱,确定共有指纹峰,并选用两种相似度计算方法进行比较。结果关木通中含有30个特征指标成分,初步建立了30个共有峰为特征指纹信息的HPLC-DAD-ESI/MS指纹图谱。24批被测样品的指纹图谱整体相似度在0.871～0.998,各产地关木通之间相似性良好。结论方法准确可靠,重现性好,可应用于关木通的品质评价和质量控制。 |
| 关 键 词： | 关木通 指纹图谱 HPLC-DAD-ESI/MS |
| 收稿时间： | 2008-06-10 |
HPLC-DAD-ESI/MS Fingerprint of Aristolochia manshuriensis |
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| FAN Xia-lei,DING Yi-bing,FENG You-long and SHANG Shu. HPLC-DAD-ESI/MS Fingerprint of Aristolochia manshuriensis[J]. Chinese Traditional and Herbal Drugs, 2008, 39(12): 1883-1887 | |
| Authors: | FAN Xia-lei DING Yi-bing FENG You-long SHANG Shu |
| Affiliation: | China Pharmaceutical University, Nanjing 210009, China;Jiangsu Institute for Drug Control, Nanjing 210008, China;Medical School of Nanjing University, Nanjing 210008, China;Jiangsu Institute for Drug Control, Nanjing 210008, China;Jiangsu Institute for Drug Control, Nanjing 210008, China;China Pharmaceutical University, Nanjing 210009, China |
| Abstract: | Objective ?To establish theanalytical method forfingerprintof Aristolochia manshuriensis?bv HPLC-DAD-ESI/MS,whichcan be used as the basis forqualitycontroI of thedrugand for the further?studies on?kidney toxicitymetabolite.Methods ?SamplesA.manshuriensis from different habitats were?extracted by 75%methanol and analyzed by HPLC-DAD-ESI/MS,whose chromatographic fingerprints?were established.TWO ways tOcalculate thesimilaritywere selected tOcomparethe Results by determining?the commonpeaks.Results ?There were 30 main characteristiccomponentsin A.rnanshuriensis.The?HPLC-DAD-ESI/MS fingerprint of the 30 common peaks was established preliminarily.The samplesof?A.manshuriensis from different habitats was found having a good similarity,andtherangeof similarities?for 24 balches of A.manshuriensiswere 0.871-O.998.Conclusion ?The method iS reliable,accurate,and?ofgood stability.andcan be used for thequalitycontrol andvarietyidentification of A.manshuriensis. |
| Keywords: | Aristolochia manshuriensis Kom. fingerprint HPLC-DAD-ESI/MS |
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