穿孔素介导的细胞毒效应在病毒性心肌炎小鼠发病中的作用

目的探讨穿孔素（ＰＦＰ）介导的细胞毒效应在病毒性心肌炎（ＶＭＣ）发病中的作用。方法４０只小鼠等分为ＣＶＢ３感染组及非感染组；所有小鼠于感染后第１０天处死并取其心脏；采用免疫组化技术检测心肌浸润细胞中ＰＦＰ的表达，原位杂交方法检测心肌中ＣＶＢ３ＲＮＡ；半定量分析ＰＦＰ抗原及ＣＶＢ３ＲＮＡ水平，并分析ＰＦＰ抗原水平与ＣＶＢ３复制及心肌病变程度的关系。结果ＰＦＰ抗原阳性信号指数与ＣＶＢ３ＲＮＡ杂交阳性信号指数呈显著负相关（ｒ＝－０．８２，Ｐ＜０．０５），与心肌病变积分呈显著正相关（ｒ＝０．９１，Ｐ＜０．０５）。结论ＰＦＰ介导的细胞毒效应在ＶＭＣ发病中可能具有抗病毒及致心肌损害双重作用。

Experimental Study on Perforin mediated Cytotoxicity on the Development of Viral Myocarditis

Purpose To clarify effect of perforinmediated cytotoxicity in the development of murine acute myocarditis. Methods Forty mice were equally divided into experimental group(CVB3 infected mice) and control group (non infected mice).The mice were killed and their hearts were removed 10 days after infection. The expression of perforin in infiltrated cells of myocardium was determined by immunohistochemistry. CVB3 RNA in myocardium was detected by in situ hybridization. In addition,the levels of perforin antigen and CVB3 RNA were semiquantitatively analyzed, and the relationship of the perforin expression level with the degrees of CVB3 replication and myocardial lesions was investigated. Results The fraction of perforin antigen positive signal show significantly correlation with that of CVB3 RNA negative signal( r=-0.82,P<0.05) and demonstrated significantly positive correlation with myocardial histopathological score ( r=0.91,P<0.05 ). Conclusions Perforin mediated cytotoxicity might play an important role in limiting virus replication and promoting cardiac damages.