青龙衣毒性作用及体外抗肿瘤作用的实验研究

目的 :青龙衣提取物及其分离成分的毒性及体外抗肿瘤作用。方法 :采用常规小鼠急性毒性试验方法 ,MTT [3-(4 ,5-dimethylthiazol -2 yl)-2 ,5-diphenylterazolium bromide ,MTT]法和SRB(sulforhodamin B)法对青龙衣提取物及其分离成分的抗肿瘤作用进行了初步筛选。结果 :除小鼠腹腔注射青龙衣氯仿萃取物LD₅₀ 为575.38mg·kg^-1和青龙衣醋酸乙酯萃取物为1303.59mg·kg^{-1,青龙衣总提取物和青龙衣石油醚萃取物、正丁醇萃取物和水萃取物小鼠口服及腹腔注射LD50 均大于 5g·kg-1。醋酸乙酯粗提部分对人白血病细胞株HL60在100 μg·mL-1时 ,细胞抑制率 <50 % ,在 100μg·mL-1剂量下 ,青龙衣的氯仿粗提部分和醋酸乙酯粗提部分对白血病细胞株HL6 0、人胃癌细胞株BGC823及人宫颈癌细胞株Hela细胞的抑制率均>50% ,其IC₅₀ (生长抑制 ) <100μg·mL-1。结论 :青龙衣氯仿提取物和醋酸乙酯提取物具有一定的抗肿瘤作用。}

Study on the acute toxicity experiment of mice and anti-tumor function in vitro of the qinglongyi

OBJECTIVE: To study the anti-tumor effect and acute toxicity in vitro of the separation compositions from qinglongyi. METHOD: The conventional acute toxicity experiments of mice, the MTT [3-(4,5-dimethylthiazol-2 yl)-2,5-diphenylterazolium bromide, MTT] and the SRB (sulforhodamin B) were used to make preliminary selection to qinglongyi and its separation compositions. RESULT: The half-deadly dose (LD50, the half-lethiferous dose) of the chloroform separation composition in qinglongyi was 575.38 mg x kg(-1) (i.g.), and of the acetic ether separation compositions in qinglongyi was 1303.59 mg x kg(-1) (i.g.). From the other parts of separation compositions in qinglongyi, the LD50 were more than 5 g x kg(-1). When acetic ether separation composition of qinglongyi was at 100 microg x mL(-1) the growth inhibitory rate (GIR) was < 50% to the leukaemia cell HL-60 of human. When chloroform separation composition and the acetic ether separation compositions of qinglongyi was at 100 mg x mL(-1), GIR was 52% to the leukaemia cell HL-60, the gastric carcinoma cell BGC-823 and the cervical carcinoma cell Hela of human. CONCLUSION: The separation composition of the chloroform and the acetic ether from qinglongyi have obvious, anti-tumor effect.