| 荧光共振能量转移技术及其在药物定量分析中的应用 |
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| 引用本文： | 王晓梅,王运庆,叶超,胡育筑. 荧光共振能量转移技术及其在药物定量分析中的应用[J]. 药学进展, 2009, 33(7): 305-310. DOI: 10.3969/j.issn.1001-5094.2009.07.003 |
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| 作者姓名： | 王晓梅 王运庆 叶超 胡育筑 |
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| 作者单位： | 1. 中国药科大学分析化学教研室,江苏,南京210009
2. 中国药科大学分析化学教研室,江苏,南京210009;药物质量与安全预警教育部重点实验室,江苏南京210009 |
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| 摘 要： | 荧光共振能量转移技术作为一种高效的光学“分子尺”，在生物医学、免疫分析以及核酸检测等方面已有广泛的应用，近年来又被用于药物的定量分析。综述荧光共振能量转移技术的原理和特点以及在药物定量分析中的应用。
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| 关 键 词： | 荧光共振能量转移 药物分析 定量分析 |
FRET Technique and Its Application in Pharmaceutical Quantitative Analysis |
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| WANG Xiao-mei,WANG Yun-qing,YE Chao,HU Yü-zhu. FRET Technique and Its Application in Pharmaceutical Quantitative Analysis[J]. Progress in Pharmaceutical Sciences, 2009, 33(7): 305-310. DOI: 10.3969/j.issn.1001-5094.2009.07.003 |
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| Authors: | WANG Xiao-mei WANG Yun-qing YE Chao HU Yü-zhu |
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| Affiliation: | WANG Xiao-mei, WANG Yun-qing, YE Chao, HU Yü-zhu (1. Department of Analytical Chemistry, China Pharmaceutical University, Nanfing 210009, China; 2. Key Laboratory of Drug Quality Control and Pharmacovigilance Affiliated to Ministry of Education, China Pharmaceutical University, Nanfing 210009, China) |
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| Abstract: | Fluorescence resonance energy transfer （FRET） technique has broad applications in the field of biomedicine, immunoassay, nucleic acid detection and so on. Recently, FRET has been introduced to pharmaceutical quantitative analysis. In this article, the theory and characteristics, and the related applications in pharmaceutical quantitative analysis of FRET technique were described. |
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| Keywords: | fluorescence resonance energy transfer pharmaceutical analysis quantitative analysis |
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