芍药苷大鼠在体肠吸收动力学的研究

 目的分别考察芍药苷在大鼠各肠段的吸收动力学特征及不同药物浓度对其的影响。方法采用大鼠在体肠吸收实验方法,用HPLC对循环液中的芍药苷进行分析。结果在4.0～40.0 mg·L^-1内芍药苷的吸收量与质量浓度成线性关系,K_a值基本保持不变;各肠段的吸收速率无显著性差异,十二指肠、空肠、回肠、结肠的Ka值分别为(0.019 19±0.004 53),(0.014 54±0.002 33),(0.014 72±0.001 89),(0.014 30±0.003 72)h^-1。结论芍药苷在肠道的吸收呈现一级动力学过程,且吸收机制为被动扩散;芍药苷在整个肠道均有吸收,可以将芍药苷研制成缓释制剂。

Studies on Absorption Kinetics of Paeoniflorin in Rat's Intestines

OBJECTIVE To investigate the absorption kinetics of paeoniflorin at different intestine segments of rats and the influence of the drug solution concentration on the absorption kinetics.METHOD The intestine in rats was cannulated for in situ recirculation.Paeoniflorin concentration in the flux was measured by the reversed phase HPLC.RESULTS When the concentration was raised from 4.0 to 40.0 mg·L^-1,the uptake of paeoniflorin was increased linearly.Concentration had no effect on the permeability coefficient.The permeability coefficient of paeoniflorin at duodenum,jejunum,ileum and colon were(0.019 19±0.004 53),(0.014 54±0.002 33),(0.014 72±0.001 89) and(0.014 30±0.003 72)h^-1,respectively.CONCLUSION The absorption of paeoniflorin in rat′s intestine is a first-order process with the passive diffusion mechanism.Paeoniflorin can be absorbed in whole intestinal segments,so paeoniflorin sustained-released formulations can be prepared.