| 特异绒毛膜促性腺激素抗血清的制备及放射免疫测定 |
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| 引用本文： | 李受增,吕云华,吴尔若,李辉,徐一树,王海云,蒋领根. 特异绒毛膜促性腺激素抗血清的制备及放射免疫测定[J]. 生殖与避孕, 1983, 0(4) |
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| 作者姓名： | 李受增 吕云华 吴尔若 李辉 徐一树 王海云 蒋领根 |
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| 作者单位： | 北京医学院第一附属医院(李受增，吕云华，吴尔若，李辉)，中国科学院北京动物研究所(徐一树，王海云)，中国科学院北京动物研究所(蒋领根) |
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| 摘 要： | 本文报道用小剂量完整的特异绒毛膜促性腺激素(hCG-β)作为免疫原,经过较长时间的免疫产生一系列抗血清,筛选后得到一个高滴度、高亲和力、特异性好的hCG-β抗血清,并用它建立了特异的hCG 放射免疫测定法。经600个不同样品的测定,结果证明该方法能在正常生理水平下(LH 存在下)特异地测定hCG,灵敏度较hCG/LH 放射免疫测定法提高10倍以上,为10毫国际单位/毫升(lst IRP hCG)。
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PREPARATIONS OF SPECIFIC ANTISERA TO hCG AND DEVELOPMENT OF hCG SPECIFIC RADIOIMMUNOASSAY |
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| Li Shou-zeng Lu Yun-hua Wu Er-ruo Li Hui. PREPARATIONS OF SPECIFIC ANTISERA TO hCG AND DEVELOPMENT OF hCG SPECIFIC RADIOIMMUNOASSAY[J]. Reproduction and Contraception, 1983, 0(4) |
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| Authors: | Li Shou-zeng Lu Yun-hua Wu Er-ruo Li Hui |
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| Abstract: | It is here reported that a series of hCG-specific antisera have been produceed,usingsmall doses intact subunit of hCG as an antigen,and a specific radioimmunoassay(RIA)for hCgG determination aftrer long term immunizion has been established.Results onmore than 600 different samples have shoun that the RIA can recongnize hCG at normalphysiological level of LH,and the sensitivily of this assay was ten times more than theconventional hCG/LH RIA,i.e.10 mI.U./ml(lst IRP hCG). |
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| Keywords: | hCG Specific antisera Radioimmunoassay |
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