溶瘤腺病毒ＳＧ６００-ｐ５３对裸鼠胃癌移植瘤的抑制作用

目的：利用我们前期构建的溶瘤腺病毒ＳＧ６００技术平台，研究其携带野生型ｐ５３基因对裸鼠胃癌移植瘤模型抗肿瘤活性。方法：克隆ｐ５３基因全长ｃＤＮＡ，插入到溶瘤腺病毒ＳＧ６００基因组中，同时建立裸鼠胃癌移植瘤模型，体内观察ＳＧ６００-ｐ５３对胃癌移植瘤模型的抗肿瘤疗效。结果：成功构建溶瘤腺病毒ＳＧ６００-ｐ５３，裸鼠胃癌移植瘤的ｐ５３基因获增强表达。在胃癌移植瘤裸鼠模型中，经４周治疗，重组ＳＧ６００-ｐ５３抑瘤率达 ７５.５９％，优于Ａｄ-ｐ５３（４５.００％）和ＳＧ６００（５６.９５％）。结论：ＳＧ６００-ｐ５３作为一种有效的抗肿瘤新型制剂，对胃癌移植瘤有很好的特异性抗肿瘤活性，可能具有良好的临床应用前景。

Antitumor activity of the oncolytic adenovirus SG600-p53 in gastric cancer xenografts in nude mice

Objective：By using the oncolytic adenovirus SG600 constructed previously, the antitumor activity of the p53 gene- enhanced oncolytic adenovirus SG600-p53 was examined in gastric cancer xenografts in nude mice. Methods：The full-length p53 cD- NA was cloned and inserted into the genome of oncolytic adenovirus SG600. The antitumor activity of SG600-p53 was observed in a nude mice model of human gastric cancer. Results： SG600-p53 could selectively kill＇gastric cancer cells, and inhibit the growth of gas- tric cancer xenografts in nude mice, with the tumor inhibition rate of 75.59%. Condusion：SG600-p53 is a novel effective antitumor agent with better antitumor activity in gastric cancer xenografts in nude mice, and may have a splendid future in clinic.