Skp2、p27蛋白在喉鳞状细胞癌中的表达及意义

目的:探讨喉鳞状细胞癌(LSCC)中Skp2和p27蛋白的表达与患者临床病理和预后的关系以及两种蛋白之间的相关性。方法:用免疫组织化学SP法检测20例正常喉黏膜(NLT)、27例LSCC癌前病变不典型增生(LPL)和70例LSCC组织中Skp2和p27蛋白的表达情况。结果:Skp2蛋白在NLT、LPL和LSCC中的阳性表达率分别为10.00%(2/20),37.04%(10/27)和41.43%(29/70),后两者的阳性率显著高于前者(均P<0.05)。Skp2蛋白表达与临床分期及淋巴结转移显著正相关(P<0.05)。p27蛋白在NLT和LSCC中阳性率分别为85.00%(17/20),48.57%(34/70),两者比较,差异有统计学意义(P<0.01)。p27蛋白表达与颈淋巴结转移呈负相关(P<0.05)。Skp2蛋白在LSCC中表达与p27蛋白呈负相关(P<0.05)。Skp2表达阳性而p27表达阴性的LSCC患者生存率明显低于其他患者(P<0.05)。结论:Skp2可能通过对细胞周期抑制因子p27蛋白的降解而参与LSCC的发生、发展,联合检测Skp2及p27蛋白的表达对预测LSCC患者的预后具有重要意义。

Expression of Skp2 and p27 proteins in laryngeal squamous cell carcinoma and their significances

Objective:To investigate the expression of Skp2 and p27and their association with clinical ,histologic characteristics and prognosis in laryngeal squamous cell carcinoma(LSCC),the correlation between expression of Skp2 and p27 in LSCC.Method:Skp2 protein and p27 protein were examined by using immunochistochemical SP staining method in 20 cases of normal laryngeal tissues(NLT),27cases of laryngeal precancerous lesion(LPL) and 27 cases of LSCC.Result:The positive rate of Skp2 in NLT,LPL and LSCC were10.00%(2/20),37.04%(10/27) and 41.43%(29/70), respectively.The positive rates in the last two groups were higer than the first one. The Skp2 protein expression in LSCC was positively associated with the clinical stage and lymph node metastasis.The positive rate of p27 protein in NLT and LSCC were 85.00%(17/20) and 48.57%(34/70),respectively,and significant difference was found between two groups.The p27 protein expression was inversely associated with lymph node metastasis.Skp2 was inversely associated with expression of p27 in LSCC.The survival rate of patients with both Skp2 positive expression and negative expression of p27 was significantly lower than that of other cases.Conclusion:Skp2 expression might play an important role in the development and progression of LSCC via cyclin-depended kinase inbitor p27 proteolysis,and the combined examination of Skp2 and p27 expression have a great significance in estimating prognosis of patients with LSCC.