小鼠颌下腺组织培养上清液促进粒-单系和巨核系造血的实验研究

目的　证实颌下腺是否合成影响造血的细胞因子 ,并探讨其对粒 -单系和巨核系造血祖细胞增殖分化有何影响 ,为重新认识颌下腺的生理功能提供实验证据。方法　采用造血祖细胞体外培养和流式细胞术检测 CD13膜抗原及细胞周期 ,研究小鼠颌下腺组织培养上清液 (SGCM)对小鼠粒 -单系和巨核系造血发生的影响。结果　 SGCM对粒 -单系祖细胞 (CFU- GM)集落生长有明显促进作用 ,能明显促进巨核系造血祖细胞 (CFU- Meg)集落生成 ,而且雄性 SGCM刺激活性高于雌性 SGCM,IL- 3与雌性 SGCM有叠加作用 ,贫血雄性 SGCM对 CFU-GM和 CFU- Meg的刺激活性高于正常小鼠 ,SGCM能促进小鼠骨髓细胞进入增殖活跃期 (S/G2 )。结论　颌下腺能合成与粒 -单系、巨核系造血相关的造血因子。提示颌下腺与造血调控可能有关

The Murine Submandibular Gland Conditioned Medium Induce the Development

Objective Our purpose is to determine whether murine submandibular gland produce hematopoietic growth factors or not and which hematopoietic growth factor are produced by murine submandibular gland.Methods By using the techniques of culture of hematopoietic progenitor cells(CFU GM, CFU Meg) ex vivo, flow cytometry, the effects of murine submandibular gland conditioned medium(SGCM) on the devlopment of CFU GM and CFU Meg in mice was studied. Results SGCM can stimulate the development of CFU GM and CFU Meg (without any exogeneous HGF), almost no colony in control (without any exogeneous HGF and SGCM); the promoting activity of male SGCM is higher than female SGCM's in augmenting colony formation of CFU Meg ( P <0.05), there is no difference of CFU GM( P >0.05). The stimulating activity of anemic mice SGCM is higher normal mice SGCM's ( P <0.05).IL 3 can collaborate with female SGCM to stimulate proliferation of CFU Meg. SGCM can promote bone morrow cell entiring the active proliferative phase(S/G 2). Conclusion Submandibular gland can stimulate the development of CFU GM and CFU Meg by secreting hematopoietic growth factor like activities. Our study provide the experimental evidences for clarifying the relationship between submandibular gland and hematopoiesis.