疏血通注射液对脑缺血大鼠细胞凋亡的影响

目的:研究疏血通对脑缺血大鼠细胞凋亡的影响。方法:雄性Wistar大鼠72只,随机分成脑缺血组、脑缺血+疏血通治疗组、假手术对照组,每组24只,采用大脑中动脉栓塞方法,制作脑缺血模型,疏血通组腹腔注射疏血通注射液0.2ml/kg,脑缺血组及假手术对照组腹腔注射等体积生理盐水。用末端脱氧核苷酸转移酶介导的缺口末端标记法(TUNEL)检测脑组织中神经细胞凋亡。结果:脑缺血组TUNEL染色阳性细胞数显著增多,并随时间的延长而加重,第3天达高峰。脑缺血+疏血通治疗组各时间点与脑缺血组比较TUNEL染色阳性细胞数明显减少(P<0.01)。结论:疏血通能够明显抑制脑缺血大鼠神经细胞凋亡。

Effects of Shuxuetong injection on cell apoptosis of rats with cerebral ischemia

Objective: To study the effect of Shuxuetong injection on cell apoptosis of rats with cerebral ischemia. Methods: Seventy-two male Wistar rats were randomly divided into three groups: cerebral ischemia group, treatment group, and control group. Each group had 24 rats. Cerebral ischemia model was performed by embolisming middle cerebral artery. The rats of treatment group were injected with Shuxuetong 0.2 ml/kg. The rats of cerebral ischemia group and control group were injected with physiological saline. The nerve cell apoptosis of cerebral tissue was detected by TUNEL test mediated with deoxynucleotidyl transferase terminal. Results: The number of TUNEL positive cells was significant increased with the time prolonging in cerebral ischemia group and obviously decreased in treatment group (P < 0.01). Conclusion: Shuxuetong injection can obviously inhibit the nerve cell apoptosis of rats with cerebral ischemia.