四环素-雌酮和雌酮作用去势大鼠的骨形态计量学对照研究

目的比较四环素－雌酮和雌酮对卵巢切除（ＯＶＸ）大鼠股骨远端骨干骨形态参数的影响。方法２０只雌性大鼠随机分成４组,每组５只：四环素－雌酮治疗组、雌酮治疗组、ＯＶＸ组和假手术组,建立ＯＶＸ大鼠骨质疏松动物模型,药物喂养１３周后处死,用骨形态计量学方法研究四环素－雌酮和雌酮对骨形态和动力学参数的影响。结果与假手术组相比,四环素－雌酮组和雌酮组骨小梁的连接性均明显地高于假手术组（Ｐ＜０．０５）。与ＯＶＸ组相比,四环素－雌酮和雌酮组四环素标记表面和类骨质表面均明显增加,四环素－雌酮组的结果又明显高于雌酮组,更明显高于其它两组（Ｐ＜０．０５）。结论四环素－雌酮和雌酮可以明显地加强骨小梁的连接性,四环素－雌酮提高骨激活频率的作用优于雌酮。

Comparative study of tetracycline-estrone and estrone effects on bone histomorphometric parameters in ovariectomized rats

Objective To compare the effects of tetracycline estrone (TE) and estrone (E) on bone histomorphometric parameters of femoral distal diaphysis in ovariectomized (OVX) rats. Methods Twenty female rats were randomly allocated into four groups, 5 rats in each: tetracycline estrone (TE), estrone (E), OVX and sham operation (S). The OVX rats were used as a model for osteoporosis. After being fed with TE or E for 13 weeks, all rats were sacrificed. The effects of TE and E on bone microarchitecture and dynamics were studied by bone histomorphometry. Results The histomorphometric data showed that the connectivity of trabecular bone in TE and E groups was significantly improved in comparision with that in S group ( P <0.05). The dynamic data indicated that in TE and E group, the tetracycline labelled and osteoid surfaces were remarkably increased in comparision with those in OVX group, especially the data in TE group were significantly higher than those in E group and other two groups ( P <0.05). Conclusions The connectivity of trabecular bone could be significantly improved by both TE and E. The activation frequency seemed to be higher in TE group than that in E group.