精子质量和数量对体外受精率,卵裂率和卵裂胚胎的影响

目的观察精子质量和数量对体外受精－胚胎移植（ＩＶＦ－ＥＴ）的卵细胞受精、卵裂和卵裂胚胎的影响。方法观察常规ＩＶＦ－ＥＴ９２个周期的精液和授精精子的密度和活动力与孕卵细胞分裂的关系。结果对４８１个成熟卵及２７３个未成熟卵的观察表明,快速直线运动（ａ级）精子和快速非直线或慢速直线运动（ｂ级）精子的密度下降,使成熟卵和未成熟卵的细胞受精率和受精卵的卵裂率明显下降;ａ级和ｂ级精子的密度增高,可明显降低形态不良卵裂胚胎的比率。结论精子的密度和活动力对体外受精过程非常重要。

Influences of sperm quality and quantity on fertilization, cleavage rates and quality of embryos in in-vitro fertilization

OBJECTIVE: To examine the influences of the quality and quantity of seminal sperm and inseminational sperm on in-vitro fertilization (IVF) outcomes. METHODS: chi-square tests were used to analyze the impacts of both semen and inseminated sperm concentrations and percentages of different grade motile sperm on the fertilization rates in 481 matured and 273 immatured oocytes and cleavage rate percentage of embryo with low quality in embryos obtained from 92 IVF-embryo transfer cycles. RESULTS: The fertilization rates of both kinds of oocytes and cleavage rate of fertilizaed matured oocyte significantly decreased (P < 0.05) as the concentrations and percentages of grade a and b sperms in semen reduced. The rates of embryos with abnormal morphology were significantly dropped when the above sperm parameters increased (P < 0.05). Meanwhile, it was also found that the concentration and motility of inseminated sperm could also affect the above three IVF parameters significantly (P < 0.05). CONCLUSION: Highly active semen and inseminated sperm play important roles not only in fertilization, but also in cleavage and embryo development during IVF procedure.