热性惊厥患儿血清细胞因子水平的变化及临床意义

目的 了解6月～2岁单纯性和复杂性热性惊厥患儿血清IFN-α，IL-8和TNF-α水平的异常变化。方法 应用ELISA法检测64例单纯性热性惊厥和52例复杂性热性惊厥患儿血清IFN-α，IL-8和TNF-α的含量，并对IL-8和TNF-α进行了相关性研究。结果 单纯性热性惊厥组血清TFN-α，IL-8和TNF-α的含量分别为467.68±112.46 ng/L，74.38±18.74 ng/L和812.36±232.38 ng/L，其含量明显高于正常对照组（P﹤0.01），也明显高于单纯性热性惊厥组（P﹤0.01）。IL-8和TNF-α在这两组疾病中分别呈正相关（r1=0.565，r2=0.64，P ﹤0.01）。结论 ①在单纯性热性惊厥和复杂性热性惊厥中，细胞因子明显增加，在增强机体免疫系统抗感染的同时，也激发了免疫性炎症反应，对组织细胞可能产生损伤。②IL-8和TNF-α在两组疾病中形成一对辅助因子，并参与了整个病理过程。

Variations of serum cytokines and its clinical significance in children with febrile convulsion.

Objective The purpose of the study was to investigate the variations of serum interferon-α (IFN-α),interleukin-8(IL-8)and tumor necrosis factor-α (TNF-α)in children aged from 6 month to 2 years with simple or complex febrile convulsion.Methods The serum IFN-α,IL-8 and TNF-α levels in 64 children cases of simple febrile convulsion and 52 children cases of complicated febrile convulsion were detected by Enzyme Linked Immunosorbent Assay (ELISA) and the relationship between serum IL-8 and TNF-α levels was studied. Results In the group of simple febrile convulsion, the levels of serum IFN-α, IL-8 and TNF-α were 467.68±112.46 ng/L, 48.74±16.5 ng/L and 578.24±168.46 ng/L, respectively, which were obviously higher than those in the control group (P<0 01). The levels of serum IFN-α,IL-8 and TNF-α in the group of complicated febrile convulsion were 584.56±124.24 ng/L,74.38±18.74 ng/L and 812.36±232.38 ng/L, respectively, which were significantly higher than those in control group (P<0 01) and simple febrile convulsion group ( P < 0 01 ). There was a positive correlation between serum IL-8 and TNF- α in simple or complicated febrile convulsion ( r 1=0.565,r 2 =0.64,P<0 01). Conclusions ①In cases of simple or complicated febrile convulsion, serum IFN- α, IL-8 and TNF-α levels are increased obviously. It can enhance body's immunity to infection, on the other hand,it may irritate immunological inflammatory reaction, thus causing tissue and cell damages. ② IL-8 and TNF- α may cooperate in febrile convulsion and be involved in the whole pathological process.