丙戊酸钠对大鼠坐骨神经损伤后脊髓运动神经元的保护作用

目的:观察丙戊酸钠对鼠坐骨神经切断后脊髓运动神经元凋亡及Bcl-2基因表达的影响。方法:采用清洁级SD大鼠30只,行右坐骨神经横形切断后即刻原位吻合术,制成周围神经损伤模型后,随机分为丙戊酸钠口服组(A组)、生理盐水对照组(B组)。分别于术后1,4,7,14,28 d取材,应用TUNEL法检测细胞凋亡数,用免疫组化技术检测脊髓运动神经元Bcl-2的表达,并对脊髓凋亡细胞以及阳性运动神经元进行计数。结果:坐骨神经切断后4,7,14 d,实验组脊髓运动神经元Bcl-2吸光度明显高于对照组(P<0.05);坐骨神经切断后7,14 d,实验组脊髓神经元凋亡率明显低于对照组(P<0.05)。结论:丙戊酸钠对鼠坐骨神经损伤后脊髓神经元具有保护作用。

Effect of Valproic Acid on Motor Neurons in Spinal Cord After Sciatic Nerve Injury in Rats

Objective: To observe the effect of valproic acid(VPA) on bcl-2 expression and motor neuronal apoptosis in spinal cord of rats following sciatic nerve transection.Methods: Thirty SD rats were divided into VPA group and control group randomly according to the treatment after operation of sciatic nerve transaction.The specimens were taken at 7,14 and 28 d after operation.Bcl-2 expression levels in spinal cord were assayed with immunohistochemistry SP method and numbers of motor neurons apoptosis with the method of TUNEL.The apoptosis neurons and positive motor neurons were counted.Results: Bcl-2 expression at 4,7 and 14 d were obviously more intensive in VPA group than in control group(P<0.05);while the apoptosis rate of spinal cord motor neurons at 7 and 14 d was obviously lower in VPA group than in control group(P<0.05).Conclusion: VPA can increase expression of bcl-2 in spinal cord and reduce neuronal apoptosis following sciatic nerve injury in rats,and has obvious protective effect on motor neuron in spinal cord of rats.