| 高效液相色谱法测定莪术中吉马酮的含量 |
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| 引用本文： | 谢永忠,夏泉. 高效液相色谱法测定莪术中吉马酮的含量[J]. 中国药房, 2005, 16(6): 461-462 |
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| 作者姓名： | 谢永忠 夏泉 |
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| 作者单位： | 1. 安徽黄山市人民医院,黄山市,245000
2. 安徽医科大学附属第一医院,合肥市,230022 |
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| 摘 要： | 目的 :建立以高效液相色谱法测定莪术中吉马酮含量的方法。方法 :色谱柱为C18,流动相为乙腈 -水 (梯度洗脱 ) ,流速为1 .0ml/min ,检测波长为214nm。结果 :吉马酮检测浓度在0. 95～285μg/ml范围内线性关系良好 (r=0 .9999) ,平均回收率为97.89 % (RSD=2.52 % ,n=5)。结论 :本方法简便、可靠 ,可用于莪术药材的含量测定和质量控制。
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| 关 键 词： | 高效液相色谱法 莪术 吉马酮 含量测定 |
| 文章编号： | 1001-0408(2005)06-0461-02 |
| 修稿时间： | 2004-07-19 |
Content Determination of Germacrone in Rhizoma Curcuma by HPLC |
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| Xie Yongzhong,XIA Quan. Content Determination of Germacrone in Rhizoma Curcuma by HPLC[J]. China Pharmacy, 2005, 16(6): 461-462 |
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| Authors: | Xie Yongzhong XIA Quan |
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| Abstract: | OBJECTIVE:To determine the content of germacrone in rhizoma curcuma by HPLC. METHODS: C18 was the chromatographic column, the mobile phase was acetonitrile and water(gradient elution) with a flow rate of 1.0ml/min, the detection wavelength was at 214nm. RESULTS:The linear range of germacrone was 0.95～285μg/ml (r=0.9 999),the mean recovery rate was 97.89%(RSD=2.52%,n=5).CONCLUSION:The method is simple and reliable and which can be used for the content determination and the quality control of rhizoma curcuma. |
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| Keywords: | HPLC Rhizoma curcuma Germacrone Content determination |
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