沙利度胺联合顺铂对A549肺腺癌细胞株的体外实验

目的观察沙利度胺与顺铂（DDP）联合应用对A549人肺腺癌细胞凋亡的影响,观察沙利度胺对肺腺癌A549细胞VEGF、BFGF蛋白表达的影响。方法采用PI单染法流式细胞检测细胞凋亡率;采用免疫细胞化学方法检测沙利度胺处理后肺腺癌A549细胞VEGF、BFGF蛋白表达的变化。结果在一定浓度范围内,沙利度胺联合DDP处理组细胞凋亡率明显高于单药组;沙利度胺（7.5mg/L）作用于人肺腺癌A549细胞后,处理组的VEGF、BFGF蛋白表达与未加药组比较差异显著。结论沙利度胺DDP联合,可增加A549人肺腺癌细胞的凋亡率;沙利度胺可抑制A549人肺腺癌细胞VEGF、BFGF的蛋白表达。

Thalidomide Combined with Cisplatin on Human Lung Carcinoma Cell Line A549 in Vitro

Objective To observe the effect of thalidomide combined with DDP on apoptosis of human lung carcinoma cell line A549,and observe the effect of thalidomide on VEGF,BFGF protein expression in A549 cells.Methods Flow cytometry with propidium iodide（PI） staining was used to detect apoptosis rate.Immunocytochemistry method was used to detect the changes of VEGF,BFGF protein expression in lung carcinoma A549 cells modified by thalidomide.Results The apoptosis rate of cells modified by thalidomide and DDP was higher than that of cells modified by single drug in certain concentration range.The VEGF,BFGF protein expression of A549 cell modified by thalidomide had significant difference compared with cells without drug.Conclusions Thalidomide combined with cisplatin can reduce the apoptosis rate of human lung carcinoma cell line A549.Thaldomide can obviously inhibit the VEGF,BFGF protein expression of human lung carcinoma cell line A549.