PRAME mRNA和SOX9 mRNA在肺腺癌中的表达及临床意义

目的 探讨黑色素瘤特异性抗原(PRAME)mRNA和性别决定基因盒9(SOX9)mRNA在肺腺癌中的表达及临床意义.方法 选取2015年2月至2018年4月于该院接受手术治疗的73例肺腺癌患者为研究对象,术中收集肺腺癌癌组织及癌旁正常组织,采用实时荧光定量PCR检测不同组织中PRAME mRNA、SOX9 mRNA的相...

Expression of PRAME mRNA and SOX9 mRNA in lung adenocarcinoma and its clinical significance

Objective To investigate the expression and clinical significance of preferentially expressed antigen of melanoma(PRAME)mRNA and sex-determining region Y box 9(SOX9)mRNA in lung adenocarcinoma.Methods Totally 73 patients with lung adenocarcinoma who were treated in the hospital from February 2015 to April 2018 were selected as the study objects.The tissues of lung adenocarcinoma and adjacent normal tissues were collected during the operation.The relative expression levels of PRAME mRNA and SOX9 mRNA in different tissues was detected by real-time quantitative PCR,and the relationship between the expression of PRAME mRNA and SOX9 mRNA in the focus tissues of patients with lung adenocarcinoma and the clinicopathological characteristics was analyzed.Spearman correlation was used to analyze the correlation between the expression of PRAME mRNA and SOX9 mRNA.The relationship between the expression of PRAME mRNA and SOX9 mRNA and the prognosis of patients with lung adenocarcinoma was analyzed.Cox regression model was used to analyze the factors affecting the prognosis of patients with lung adenocarcinoma.Results Compared with adjacent normal tissues,the relative expression level of PRAME mRNA in lung adenocarcinoma tissue was lower(P<0.05),and the relative expression level of SOX9 mRNA was higher(P<0.05).The expression of PRAME mRNA and SOX9 mRNA was not correlated with gender,age and tumor size(P>0.05),but correlated with pathological grade,clinical stage and lymph node metastasis(P<0.05).Spearman correlation analysis showed that the expression of PRAME mRNA was negatively correlated with SOX9 mRNA in lung adenocarcinoma(P<0.05).Compared with good prognosis group,the relative expression level of PRAME mRNA was lower and the relative expression level of SOX9 mRNA was higher in poor prognosis group(P<0.05).COX regression model analysis showed that low expression of PRAME mRNA,high expression of SOX9 mRNA,poor differentiation,stageⅢclinical stage and lymph node metastasis were independent risk factors for the prognosis of patients with lung adenocarcinoma(P<0.05).Conclusion The abnormal expression of PRAME mRNA and SOX9 mRNA may be involved in the occurrence and progression of lung adenocarcinoma,and the two are negatively correlated.It may provide a reference for the condition evaluation and prognosis evaluation of lung adenocarcinoma and targeted therapy.