妊娠高血压综合征患者MTHFR基因C677T多态性与Hcy及肾功能指标的相关性研究

目的探讨妊娠高血压综合征(简称妊高征)患者5,10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与同型半胱氨酸(Hcy)、肾功能指标的相关性。方法纳入妊高征患者86例(疾病组,根据疾病严重程度,进一步分为妊娠高血压组、轻度子痫前期组、重度子痫前期组3个亚组),同时纳入健康妊娠女性200例(正常妊娠组)和健康非妊娠女性150例(健康对照组),采集研究对象的血液标本进行Hcy、尿酸(UA)、肌酐(Cr)及尿素氮(BUN)水平检测,并进行分析。此外,对86例妊高征患者的MTHFR基因进行测序,利用Chromas软件对测序结果跟健康人群进行比对,找出多态性位点进行分析。结果与健康对照组、正常妊娠组相比,疾病组Hcy、UA、Cr及BUN水平均明显升高(P<0.05),且以上指标在重度子痫前期组明显升高(P<0.05)。MTHFR C677T基因型与Hcy具有相关性(P<0.05),Hcy在TT基因型患者中的水平高于CC基因型和CT基因型患者(P<0.05)。结论Hcy及肾功能指标可作为妊高征辅助诊断及病情监测的重要指标,MTHFR基因C677T多态性的检测可为妊高征患者病情严重程度及预后判断提供一定依据,为发现妊高征高危人群提供新思路。

Correlation between MTHFR gene C677T polymorphism with homocysteine and renal function indicators in patients with hypertensive disorder complicating pregnancy

Objective To investigate the correlation between MTHFR gene C677T polymorphism with homocysteine and renal function indicators in the patients with hypertensive disorder complicating pregnancy(HDCP).Methods Eighty-six cases of HDCP were enrolled(disease group,which was further divided into three subgroups:the HDCP group,mild eclampsia prophase group and severe eclampsia prophase group).At the same time,200 females of healthy pregnancy(normal pregnancy group)and 150 healthy females of non-pregnancy(healthy control group)were included.The blood samples were collected for detecting the levels of homocysteine(Hcy),uric acid(UA),creatinine(Cr)and urea nitrogen(BUN),and conducting the analysis.In addition,the MTHFR gene in the samples of 86 HDCP cases conducted the sequencing,then the sequencing results were compared with the healthy population by using the Chromas sequence comparison software.The polymorphic sites were found out for conducting the analysis.Results Compared with the healthy control group and normal pregnancy group,the levels of serum Hcy,UA,Cr and BUN of the disease group were significantly increased(P<0.05),moreover the above indicators in the severe eclampsia prophase group were much significantly increased(P<0.05).The MTHFR C677T genotype had the correlation with Hcy(P<0.05)and the Hcy level in the TT genotype patients was higher than that in the CC genotype and CT genotype patients(P<0.05).Conclusion Hcy and renal function indicators can serve as the important indicators of auxiliary diagnosis and disease condition monitoring of HDCP,the MTHFR gene C677T polymorphism detection provides a certain basis for judging the severity of the patients with HDCP and prognosis,which provides a new idea for finding the population of HDCP.