PBMCs中NS5ATP9、IL-12p40、IRAK-1的mRNA水平联合检测对早期胃癌的诊断价值

目的 探讨外周血单个核细胞(PBMCs)中非结构蛋白5A反式激活蛋白9(NS5ATP9)、白细胞介素-12p40(IL-12p40)、白细胞介素1受体相关激酶1(IRAK-1)的mRNA水平联合检测对早期胃癌的诊断价值.方法 将2017年11月至2019年11月该院收治的早期胃癌患者112例作为早期胃癌组,胃部良性疾病...

Diagnostic value of NS5ATP9,IL-12p40,IRAK-1 mRNA levels in PBMCs for early gastric cancer

Objective To explore the diagnostic value of the combined detection of nonstructural protein 5A transactivator 9(NS5ATP9),interleukin-12p40(IL-12p40)and interleukin-1 receptor-associated kinase-1(IRAK-1)mRNA levels in Peripheral blood mononuclear cells(PBMCs)for early gastric cancer.Methods A total of 112 patients with early gastric cancer admitted in the hospital from October 2017 to October 2019 were enrolled in the study as early gastric cancer group,during the same period 112 patients with benign gastric disease were enrolled as benign group,and 112 healthy people as the control group.PBMCs were extracted from the people of the 3 groups and the mRNA levels of NS5ATP9,IL-12p40,and IRAK-1 in those PBMCs samples were detected by real-time fluorescent quantitative PCR.The area under curve(AUC)of receiver operating characteristic curve(ROC)was used to analyze the diagnostic value of NS5ATP9,IL-12p40,IRAK-1 mRNA levels in PBMCs for early gastric cancer.Results The mRNA levels of NS5ATP9,IL-12p40 and IRAK-1 in PBMCs of patients with early gastric cancer were significantly higher than those of patients with benign gastric diseases and healthy people(P<0.05).The sensitivity of NS5ATP9,IL-12p40,and IRAK-1 mRNA levels in PBMCs to distinguish patients with early gastric cancer from patients with benign gastric diseases were 91%,87%,and 93%,and the specificities were 91%,88%,and 92%,respectively.The sensitivity of NS5ATP9,IL-12p40,and IRAK-1 mRNA levels in PBMCs used to distinguish early gastric cancer patients from healthy subjects were 96%,92%,95%,and specificity were 94%,91%,95%,respectively.The sensitivity of the combined detection of NS5ATP9,IL-12p40,and IRAK-1 mRNA in PBMCs for the diagnosis of early gastric cancer was 92.86%,the specificity was 93.30%,and the accuracy was 93.15%.Conclusion NS5ATP9,IL-12p40 and IRAK-1 mRNA in PBMCs are potential markers of early gastric cancer.