胶体金法与ELISA检测新型冠状病毒IgM和IgG抗体的临床诊断价值比较

目的 通过胶体金法与酶联免疫吸附测定(ELISA)检测新型冠状病毒(SARS-CoV-2)特异性抗体免疫球蛋白(Ig)M和IgG,评价2种方法在检测SARS-CoV-2特异性抗体中的诊断价值.方法 收集2020年1-2月在该院住院的患者81例,根据相关标准分为新型冠状病毒肺炎(COVID-19)确诊患者组38例,疑似患...

Comparison of clinical diagnostic value of colloidal gold and ELISA for detecting severe acute respiratory syndrome coronavirus 2 IgM and IgG antibodie

Objective To detect immunoglobulin(Ig)M and IgG specific to the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)by colloidal gold method and enzyme-linked immunosorbent assay(ELISA),and evaluate the diagnostic value of two methods in the detection of SARS-CoV-2 specific antibodies.Methods A total of 81 patients who were hospitalized in this hospital from January to February 2020 were collected.According to relevant standards,they were divided into 38 confirmed patients of corona virus disease-19(COVID-19)and 43 suspected patients.The colloidal gold method and ELISA were used to detect SARS-CoV-2 IgM and IgG antibody in all subjects,and the sensitivity and specificity of the two detection methods were calculated.Theχ² test was used to compare the differences in the detection results of suspected patients,and the consistency of the two detection methods was analyzed.Results The sensitivity of ELISA to detect SARS-CoV-2 IgM and IgG antibody were 55.26%(21/38),60.53%(23/38),respectively,and the both specificity were 97.67%(42/43).The sensitivity of the colloidal gold method to detect SARS-CoV-2 IgM and IgG antibody were 63.16%(24/38),76.32%(29/38),and the both specificity were 93.02%(40/43).The positive rate of combined detection of SARS-CoV-2 specific antibody IgM and IgG>70%.There was no statistically significant difference between the two methods for detecting IgM and IgG antibodies.methods for detecting IgM and IgG antibodies(P>0.05).Conclusion In the process of serum antibody detection in patients with COVID-19,the colloidal gold method is simple,fast,low-cost,and has good specificity.The combined detection of specific antibodies IgM and IgG can be used as a supplementary method for nucleic acid detection.