DNA微阵列芯片法在海南地区结核病诊断及耐药性检测中的应用

目的 探讨DNA微阵列芯片法在海南地区结核病诊断及耐药性检测中的应用.方法 采用抗酸杆菌涂片法、罗氏培养法、比例法药敏试验及DNA微阵列芯片法对海南地区的2069例疑似结核病患者痰标本进行检测,并对结核分枝杆菌检出率、耐药性及耐药基因突变特征进行分析.结果 DNA微阵列芯片法检测结核分枝杆菌检出率为明显高于罗氏培养法和...

Application of DNA microarray in diagnosis and drug resistance detection of tuberculosis in Hainan area

Objective To explore the application of DNA microarray in the diagnosis and drug resistance detection of tuberculosis in Hainan.Methods Acid fast bacilli smear method,Roche culture method,proportion method,drug susceptibility and DNA microarray method were used to to detect the sputum samples of 2069 suspected tuberculosis patients in Hainan area,and the detection rate,drug resistance and drug resistance gene mutation characteristics of Mycobacterium tuberculosis were analyzed.Results The detection rate of Mycobacterium tuberculosis by using DNA microarray was significantly higher than that by Roche culture or acid fast bacilli smear(P<0.05).Taking proportion method as the gold standard,DNA microarray method and proportion method were compared in the detection of rifampicin resistance,isoniazid resistance and multi-drug resistance of Mycobacterium tuberculosis,the difference was statistically significant(P<0.05),while there was good consistency(Kappa≥0.75).The gene mutation which lead to rifampicin resistance was mainly rpoB531(C→T),accounting for 56.3%;the gene mutation which lead to isoniazid resistance was mainly KatG315(G→C),accounting for 84.6%;the gene mutation which lead to multi-drug resistance of Mycobacterium tuberculosis was mainly rpoB531+KatG315,accounting for 54.6%.Conclusion Mycobacterium tuberculosis in sputum samples and its drug resistance of suspected tuberculosis patients in Hainan area can be detected by using DNA microarray method quickly and accurately,thereby guiding clinical medication.