| 己基可可碱对成纤维细胞的生物学影响 |
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| 引用本文： | 吕远东,罗少军,刘嘉琦,汤少明. 己基可可碱对成纤维细胞的生物学影响[J]. 中国实用美容整形外科杂志, 2002, 0(2) |
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| 作者姓名： | 吕远东 罗少军 刘嘉琦 汤少明 |
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| 作者单位： | 哈尔滨医科大学附属第一临床医学院整形烧伤科，广东医学院整形外科研究所，哈尔滨医科大学附属第一临床医学院妇产科，广东医学院整形外科研究所 黑龙江哈尔滨150001 |
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| 摘 要： | 目的 探讨己基可可碱对成纤维细胞的生物学作用及其意义。方法 通过MTS/PMS法、流式细胞术、3H-胸腺嘧啶摄入法、3H -脯氨酸摄入法的实验 ,观察了己基可可碱对体外培养的增生性瘢痕成纤维细胞的增殖活性和胶原合成的作用。结果 己基可可碱能够抑制瘢痕成纤维细胞的增殖和胶原的合成 ,并且具有明显的时间 -剂量依赖性 ,随着作用时间的延长和作用剂量的增大 ,抑制率逐渐增高。结论 己基可可碱具有抑制瘢痕成纤维细胞增殖和胶原合成的作用 ,从而达到抑制瘢痕增生的作用
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| 关 键 词： | 成纤维细胞 胶原 细胞增殖 己基可可碱 |
The biological effects of pentifylline on fibroblasts |
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| LU Yuan-dong,LUO Shao-jun,LIU Jia-qi,et al.. The biological effects of pentifylline on fibroblasts[J]. , 2002, 0(2) |
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| Authors: | LU Yuan-dong LUO Shao-jun LIU Jia-qi et al. |
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| Abstract: | Objective To observe the effects of pentifylline on fibroblasts derived from human hypertrophic scar. Methods Fibroblasts derived from human hypertrophic scar were cultured in vitro. The effects of pentifylline on cell proliferation and collagen synthesis of fibroblasts were evaluated by MTS/PMS method, flow cytometry and 3 H thymidine uptake assay and 3 H proline uptake assay. Results It demonstrated that pentifylline can inhibit cell proliferation and collagen synthesis of fibroblasts derived from pathologic scar in a time-dose dependent manner. Such effects were increased while the concentration of the drugs was raised or the given time of action was prolonged. Conclusion Pentifylline can significantly inhibits the proliferation and collagen synthesis of hypertrophic scar fibroblast in vitro, which may have some effect in ameliorate pathologic scar formation. |
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| Keywords: | Fibroblast Collagen Cell proliferation Pentifylline |
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