嗅鞘细胞对神经干细胞向胆碱能神经元分化的影响

目的：研究不同浓度嗅鞘细胞（OECs）对神经干细胞（NSCs）向胆碱能神经元分化的影响,为细胞联合移植治疗阿尔茨海默病提供理论依据。方法：分别体外培养NSCs和OECs。将5×10^4/mL的NSCs分别与1×10^4/mL、1×10^6/ mL、1×10^8/mL OECs共培养7 d,同时设立对照组（不加OECs）。用免疫细胞化学法检测胆碱乙酰化酶阳性细胞表达。结果：共培养7 d后,OECs能促进NSCs向胆碱能神经元分化,以1×10^6/mLOECs与NSCs共培养作用最明显,与对照组比较差异有显著统计学意义（P〈0.01）。结论：OECs可促进NSCs向胆碱能神经元分化。

Effects of Olfactory Ensheathing Cells on the Differentiation of Neural Stem Cells to Cholinergic Neuron

Aim：To investigate the effects of different concentration of olfactory ensheathing cells（OECs） on the differentiation of neural stem cells（NSCs） to cholinergic neurons,and provide a theoretical basis for cell transplantation for the treatment of AD.Methods：OECs and NSCs were separately cultured in vitro.5×10~4/ mL NSCs and 1×10~4/mL,1×10~6/mL,1×10~8/mL OECs were co-cultured,respectively in 7 days.NSCs in the control group were left intact.ChAT positive cells were detected with immunocytochemical method. Results：After co-cultured for 7 days,it was found OECs could promote the differentiation of NSCs into cholinergic neurons.NSCs and 1×10~6/mL OECs group was more than the other groups.The increase in the differentiation of NSCs to cholinergic neurons showed significant statistic difference compared with the control group（P0.01）.Conclusion：OECs can induce NSCs differentiate into cholinergic neurons.