A型肉毒杆菌毒素降低豚鼠鼻黏膜腺细胞乙酰胆碱酯酶浓度的研究

目的:探讨A型肉毒杆菌毒素(BTA)对豚鼠鼻黏膜腺细胞乙酰胆碱酯酶浓度的影响。方法:18只雄性豚鼠随机分为BTA组和对照组。BTA组左侧鼻腔以浸有0.2ml(10U)BTA的Merocel作用1h,对照组以生理盐水代替BTA。分别于用药后第1、2、4周全身麻醉下切取豚鼠左侧下鼻甲黏膜(每个时间点每组3只),4%多聚甲醛固定后石蜡包埋、切片,免疫组织化学染色,光镜下定量分析。结果:BTA局部应用后第1、2周豚鼠鼻黏膜腺体及腺上皮中腺细胞的乙酰胆碱酯酶浓度显著降低,与对照组比较,差异有统计学意义(均P<0.01);第4周乙酰胆碱酯酶浓度恢复正常。结论:BTA可降低豚鼠鼻黏膜腺细胞乙酰胆碱酯酶的浓度。

Botulinum toxin type A decreases the concentration of acetylcholinesterase in nasal glands of guinea pig

Objective:To investigate the effect of botulinum toxin type A(BTA)on the concentration of acetylcholinesterase in nasal glands of guinea pig.Method:Eighteen male guinea pigs were randomly divided into BTA group and control group.Merocel sponge soaked with 10 U (0.2 ml) BTA was put into left nasal cavity of guinea pig under general anesthesia for one hour in BTA group.Saline replaced BTA in control group.The inferior turbinate mucosae of guinea pig were harvested under general anesthesia at 1, 2, 4 weeks after BTA treatment (3 animals in each group). The tissues were fixed in 4% paraformaldehyde and paraffin embedded. The concentration of acetylcholinesterase was determined with immunohistochemistry staining.Result:One,two weeks after local BTA application, the expression of acetylcholinesterase in nasal glandular and canal cells decreased significantly( P < 0.01 ). It became normal at 4 weeks after BTA treatment.Conclusion:BTA can decrease the concentration of acetylcholinesterase in nasal glands of guinea pig.