基因芯片在乙肝病毒检测中的应用

目的]评价基因芯片技术在同时进行乙肝病毒基因分型及耐药突变基因检测中的准确性、敏感性和临床应用价值.方法]50例血清标本都用荧光定量PCR和基因芯片两种方法进行检测,然后对两种方法的结果进行比较,同时对基因芯片技术检测结果中乙肝病毒基因分型和耐药突变基因的情况进行分析.结果]荧光定量PCR的阳性检出率为46%.基因芯片的阳性检出率为38%,其中10例为B型,9例为C型,结合荧光定量的结果,在整个基因芯片的检测结果中检测到的最低拷贝数为1.387e3 copies/ml.19例阳性都存在拉米夫定敏感位点,共检测到3例对拉米夫定耐药的患者(2例为rt204I突变,1例为rt108M、rt204V突变).结论]基因芯片技术在同时进行乙肝病毒基因分型和耐药突变基因的检测中,结果准确、灵敏度高、通量性好,适合各大临床单位开展应用.

APPLICATION OF MICROARRAY IN DETECTION OF HEPATITIS B VIRUS

Objective To evaluate the sensitivity,accuracy and clinical application value of microarray technique in simultaneous detection of hepatitis B virus genotypes and drug resistance mutation genes.Methods50 serum samples were detected both by Fluorescent quantitive PCR and Microarray .Then results of these two methods were compared,equally,hepatitis B virus genotypes and drug resistance mutation genes detected by microarray were analyzed.ResultsPositive detection rates of FQ-PCR and Microarray were 46% and 38%,respectively. Among these positive samples detected by microarray,10 cases were genotype B,and 9 cases were genotype C. Connected with the results of FQ-PCR,the lowest copy numbers were 1.387e3 copies/ml among the those detected by microarray. Lamivudine sensitive genes were founded in all 19 positive cases and Lamivudine resistance mutation genes were detected from 3 of 19 positive cases(two cases were rt204I,one case was rt108M and rt204V).ConclusionMicroarray technique is a method with high accurate,sensitive,specificity and throughput for the detection of HBV genotypes and drug resistance mutation genes,which could be applied to be a diagnostic technique in the clinical laboratory.