67例重度烧伤患者血清铜、锌、锰、硒含量变化与SOD、GSH-Px活性关系的研究

目的 研究重度烧伤患者烧伤早、中期血清中铜、锌、锰、硒含量的变化与超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力之间的关系,为临床烧伤治疗学提供一定的理论依据.方法 随机抽取我院烧伤科新入院重度烧伤患者67例血液标本,利用原子吸收法测定其中铜、锌、锰、硒的含量,同时用南京建成生物工程研究所提供的试剂盒测定SOD、GSH-Px活力.结果 烧伤后早期铜在血液中含量有所增加,锌在血液中含量减少,锰、硒在血液中变化不大,但它们尿液中均排出增加,而T-SOD及Cu,Zn-SOD自烧伤早期即开始下降持续至病程中期;GSH-Px活力于烧伤早期开始下降持续至病程中期.结论 烧伤后早期由于各组织内氧自由基的大量产生,使SOD和GSH-Px严重消耗,含量因此下降;微量元素由于组织的破坏释放入血,尿中排泄增多,使组织不能够产生足够的抗氧化酶系,对抗炎症反应能力下降、组织修复能力下降.因此本实验对重度烧伤患者早、中期合理补充微量元素提供了一定的理论依据,对烧伤病理学、病因治疗学上提供了一定的线索.

Relationship between plasma content of Cu, Zn, Mn, Se and enzymatic activities of SOD and GSH-Px following severe burn injury

Objective To study the relationship of concentraions of trace elements and the SOD, GSH-Ps activities in plasma following severe burn injury, and provide witness for clinical treatment of trace elements disorder in burned patients. Methods The plasma and urine from 67 severely burned patients in our hospital were collected on day 2, 4, 6, 8, 10, 15, 20, 30 after hospitalization. Trace element levels were determined by atomic absorption spectrophotometry. The vital force of SOD and GSH-Px were measured in plasma with the kits provided by Nanjing Jiancheng bioengineering research institute. Results The ion concentration of Cu in serum got higher and that of Zn got lower after burn, but that of Mn, Se was of no significance. The concentration of Cu, Zn, Mn, Se in urine were increased. The enzymatic activities of T-SOD and Cu, Zn-SOD as well as GSH-Px decreased from the early period to the metaphase. Conclusion For a great deal of oxygen free radicals released by tissues, SOD and GSH-Px were consumed and their concentration decreased in early stage after burn. Due to the trace elements released into blood by destroyed tissues and their increased excretion from urine, tissues could not generate adequate antioxidase, resulting in weak ability against inflammation and poor tissue repair. Our result provides a theoretical clue for the replenishment of trace elements for severely burned patients in early and middle stage and offer a clue to pathology and etiological treatment of burn.