| 采用流式细胞仪检测艾滋病病毒1型膜蛋白小鼠免疫后IFN-γ的表达 |
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| 引用本文： | 吴岚,冯毅,张旭东,刘颖,唐海丽,李红梅,姚均,冯鑫,邵一鸣.采用流式细胞仪检测艾滋病病毒1型膜蛋白小鼠免疫后IFN-γ的表达[J].中国艾滋病性病,2002,8(4):212-215. |
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| 作者姓名： | 吴岚 冯毅 张旭东 刘颖 唐海丽 李红梅 姚均 冯鑫 邵一鸣 |
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| 作者单位： | 1. 中国疾病预防控制中心性病艾滋病预防与控制中心病毒与免疫研究室,北京,100050
2. 北京地坛医院 |
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| 基金项目： | 本研究受基础研究重大项目前期研究专项(编号:2001CCA00600)课题资助 |
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| 摘 要： | 目的通过流式细胞技术检测艾滋病病毒1型(HIV-1)Gp120膜蛋白免疫小鼠后的IFN-γ的表达。方法 构建表达HIV-1gp120基因的复制型重组痘苗病毒。与DNA疫苗联合免疫小鼠,用流式细胞仪检测小鼠脾淋巴细胞中IFN-γ的表达。结果 获得表达中国HIV-1流行株gp120基因的重组痘苗病毒rVVgp120、rVVM304,能正确表达Gp120蛋白。与DNA疫苗p120-VRC、pM304-VRC联合免疫小鼠后,用流式细胞仪检测到小鼠脾淋巴细胞能特异性的表达IFN-γ。结论 用流式细胞检测技术成功检测到免疫小鼠的脾淋巴细胞特异性的表达IFN-γ,用此方法可方便快捷地检测小鼠的细胞免疫效果。
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| 关 键 词： | 艾滋病病毒1型疫苗 细胞因子 流式细胞仪 |
| 修稿时间： | 2002年6月5日 |
Detection of IFN-γ Expression of Mice by Flow Cytometry Primed with DNA and Boosted with Recombinant Vaccinia virus Expressing Gp120 gene of Human Immunodeficiency Virus |
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| Wu Lan,Feng Yi,Zhang Xudong,Liu Ying,elal. National Center for AIDS/STD Control and Prevention. Chinese Center for Disease Control and Prevention,Beijing ,China..Detection of IFN-γ Expression of Mice by Flow Cytometry Primed with DNA and Boosted with Recombinant Vaccinia virus Expressing Gp120 gene of Human Immunodeficiency Virus[J].Chinese JOurnal of Aids & STD,2002,8(4):212-215. |
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| Authors: | Wu Lan Feng Yi Zhang Xudong Liu Ying elal National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing China |
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| Institution: | Wu Lan,Feng Yi,Zhang Xudong,Liu Ying,elal. National Center for AIDS/STD Control and Prevention. Chinese Center for Disease Control and Prevention,Beijing 100050,China. |
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| Abstract: | Objective To detect IFN-y expression of mice immunized with DNA and recombinant vaccinia expressing gp120gene of human immunodeficiency virus by using flow cytometry. Method Replication-competent recombinant vaccinia virus rVVgp120 and rVVM304 expressing gp120 and M304 gene was constructed respectively. Its immunogenicity was evaluated by testing IFN-y levels of mice primed with DNA vaccines p120-VRC, pM304-VRC and boosted with recombinant vaccinia through flow cytometry. Results The replication-competent recombinant vaccinia can virus express gp120 protein correctly. The mice primed with DNA vaccines and boosted with recombinant vaccinia virus elicited HIV-1 specific IFN-y.Conclusion Flow cytometry can detect mouse IFN-y immunized with DNA and recombinant vaccinia virus expressing gp120 gene of human immunodeficiency virus. It is a rapid method to detect the cellular immune responses. |
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| Keywords: | HIV-1 vaccine Cellular immune response Flow Cytometry |
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