| 人胚胎干细胞来源的成纤维细胞支持无动物源污染条件下的人精原干细胞生长 |
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| 引用本文： | Bin Chen,Yu-Bin Wang,Zhi-Ling Zhang,Wei-Liang Xia,Hong-Xiang Wang,Zu-Qiong Xiang,Kai Hu,Yin-Fa Han,Yi-Xin Wang,Yi-Ran Huang,Zheng Wang. 人胚胎干细胞来源的成纤维细胞支持无动物源污染条件下的人精原干细胞生长[J]. Asian journal of andrology, 2009, 11(5): 557-565,I0002. DOI: 10.1038/aja.2009.21 |
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| 作者姓名： | Bin Chen Yu-Bin Wang Zhi-Ling Zhang Wei-Liang Xia Hong-Xiang Wang Zu-Qiong Xiang Kai Hu Yin-Fa Han Yi-Xin Wang Yi-Ran Huang Zheng Wang |
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| 作者单位： | [1]Department of Urology, Shanghai Institute of Andrology, Renji Hospital, Shanghai Jiao Tong University, School ofMedicine, Shanghai 200001, China [2]Department of Obstetrics-Gynecology, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai200001, China [3]Department of Medicine, UCSF and San Francisco Vii Medical Center, San Francisco, CA 94121, USA [4]Shanghai Institute of Digestive Disease, Stem Cell Laboratory, Renji Hospital, Shanghai Jiao Tong University, School ofMedicine, Shanghai 200001, China |
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| 基金项目： | Acknowledgment This study was supported by grants from the Shanghai Municipal Population and Family Planning Commission, China （No. 2007JG06） and the Shanghai Leading Academic Discipline Project, China （No. Y0205）. |
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| 摘 要： | 精原干细胞在人出生后可以连续分裂以支持精子形成，将遗传信息传递给下一代。在本文中，我们成功地从睾丸组织中分离并鉴别人精原干细胞的一种新方法，并在人胚胎干细胞来源的成纤维细胞上扩增人精原干细胞。在这种人胚胎干细胞来源的成纤维细胞上可维持大量的人精原干细胞并且在组合生长因子，特别是胶质细胞来源的神经生长因子存在的条件下扩增至少2个月。细胞表面标志分析显示这些精原干细胞具有高水平的碱性磷酸酶的表达并保持了高水平的胚胎特异阶段抗原SSEA-1，OCT4和CD49f的表达。用逆转录PCR反应检测出OCT4，SOX3，STRA8基因的表达。这些数据清晰地表明运用人胚胎干细胞来源的成纤维细胞来支持人精原干细胞的一个新的策略，并排除了异源物种的污染。这个系统提供了新的机会去了解干细胞巢对于控制精原干细胞自我更新的调节机制，并将对于精原干细胞潜在的临床应用提供有用的精原干细胞。
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| 关 键 词： | 人胚胎干细胞来源的成纤维样细胞 精原干细胞 无异种动物污染 胚胎学 |
| 收稿时间： | 2008-09-05 |
Xeno-free culture of human spermatogonial stem cells supported by human embryonic stem cell-derived fibroblast-like cells |
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| Bin Chen Yu-Bin Wang Zhi-Ling Zhang Wei-Liang Xia Hong-Xiang Wang Zu-Qiong Xiang Kai Hu Yin-Fa Han Yi-Xin Wang Yi-Ran Huang Zheng Wang. Xeno-free culture of human spermatogonial stem cells supported by human embryonic stem cell-derived fibroblast-like cells[J]. Asian journal of andrology, 2009, 11(5): 557-565,I0002. DOI: 10.1038/aja.2009.21 |
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| Authors: | Bin Chen Yu-Bin Wang Zhi-Ling Zhang Wei-Liang Xia Hong-Xiang Wang Zu-Qiong Xiang Kai Hu Yin-Fa Han Yi-Xin Wang Yi-Ran Huang Zheng Wang |
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| Affiliation: | [1]Department of Urology, Shanghai Institute of Andrology, Renji Hospital, Shanghai Jiao Tong University, School ofMedicine, Shanghai 200001, China [2]Department of Obstetrics-Gynecology, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai200001, China [3]Department of Medicine, UCSF and San Francisco Vii Medical Center, San Francisco, CA 94121, USA [4]Shanghai Institute of Digestive Disease, Stem Cell Laboratory, Renji Hospital, Shanghai Jiao Tong University, School ofMedicine, Shanghai 200001, China |
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| Abstract: | |
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| Keywords: | human embryonic stem cell-derived fibroblast-like cells （hdFs） spermatogonial stem cells （SSCs） xeno-free culture |
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