| 己酮可可碱对腹膜纤维化形成的干预作用 |
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| 引用本文： | 龚燕梅,姜燕,曾红兵,刘晓城. 己酮可可碱对腹膜纤维化形成的干预作用[J]. 华中科技大学学报(医学版), 2008, 37(5). DOI: 10.3870/j.issn.1672-0741.2008.05.008 |
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| 作者姓名： | 龚燕梅 姜燕 曾红兵 刘晓城 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院肾内科,武汉,430030 |
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| 摘 要： | 目的 探讨己酮可可碱(PTX)对SD大鼠腹膜纤维化病变的影响和可能的机制.方法 将大鼠随机分成3组:对照组,模型组和治疗组(PTX组).用4.25%含糖腹膜透析液 脂多糖腹腔注射制作腹膜纤维化模型,即模型组;PTX组同时每日给予PTX 6 mg/kg腹腔注射.5周后行2 h腹膜平衡试验测定腹膜功能,留取血液及腹膜透析液用ELISA法测定血管内皮生长因子(VEGF)浓度,并留取壁层腹膜组织做苏木精-伊红、Masson染色,RT-PCR检测肠系膜组织VEGF mRNA表达水平.结果 ①模型组及PTX组的壁层腹膜较对照组增厚,而PTX组较模型组明显减轻;②模型组及PTX组大鼠超滤量及2 h透析液葡萄糖浓度/0 h透析液葡萄糖浓度(D2/D0)比值均较对照组减少(均P<0.05),其中模型组较PTX组明显减少;与对照组比较,模型组及PTX组大鼠透析液尿素浓度/血浆尿素浓度(Durea/Purea)比值有明显增加(均P<0.05),其中模型组较PTX组增加明显;③PTX组腹透液及血清中VEGF浓度,肠系膜组织VEGF mRNA表达较模型组明显下降(均P<0.05).结论 己酮可可碱能有效地预防腹膜纤维化,其机制可能与调节VEGF的表达有关.
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| 关 键 词： | 腹膜纤维化 己酮可可碱 血管内皮生长因子 |
Effects of Pentoxifylline on Peritoneal Fibrosis |
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| Gong Yanmei,Jiang Yan,Zeng Hongbing et al. Effects of Pentoxifylline on Peritoneal Fibrosis[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2008, 37(5). DOI: 10.3870/j.issn.1672-0741.2008.05.008 |
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| Authors: | Gong Yanmei Jiang Yan Zeng Hongbing et al |
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| Affiliation: | Gong Yanmei,Jiang Yan,Zeng Hongbing et al Department of Nephrology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030 |
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| Abstract: | Objective To investigate the effect and possible mechanism of pentoxifylline in treating peritoneal fibrosis of SD rats.Methods All rats were randomly divided into three groups:control group,model group and pentoxifylline-treated group.The rat model of peritoneal fibrosis was induced by high glucose peritoneal dialysis fluid(4.25%,Baxter) and lipopolysaccharide(LPS)(model group).In pentoxifylline-treated group,pentoxifylline(6 mg/kg) was intraperitoneally injected daily.Five weeks later the function of peri... |
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| Keywords: | peritoneal fibrosis pentoxifylline vascular endothelial growth factor |
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