巴戟天水提物对人精子膜功能氧化损伤的保护作用

目的：观察巴戟天水提物对人精子膜结构和功能氧化损伤的干预作用,探讨巴戟天治疗弱精子症男性不育的作用机制。方法：采用Percoll梯度离心法优选具有正常功能的精子悬液分为正常组,模型组,阳性药对照组（维生素C组）和巴戟天水提物小、中、大剂量组,应用次黄嘌呤-黄嘌呤氧化酶（HX-XO）体系产生活性氧（ROS）,在有氧环境下,ROS、不同剂量（0.125,0.25,0.5 g·mL^-1）的巴戟天水提物与精子悬液共同孵育后,检测精子膜脂质过氧化损伤程度,通过精子低渗膨胀试验评估精子膜功能,并与已知的抗氧化剂维生素C对照。结果：巴戟天水提物小、中、大剂量组（0.125,0.25,0.5 g·mL^-1）在相同的条件下均可提高精子悬液SOD活力,降低MDA含量,对ROS所致精子膜的氧化损伤均具有不同程度的干预作用,对精子膜功能具有一定的保护作用。其中0.125 g·mL^-1的巴戟天水提物组与维生素C组（0.25 mg·mL^-1）无明显差别,0.25,0.5 g·mL^-1的巴戟天水提物组明显优于维生素C组（P＜0.01,P＜0.001）。且巴戟天水提物的作用呈剂量依赖性,大剂量（0.5 g·mL^-1）时对精子膜功能的保护作用最显著。结论：巴戟天水提物对人精子膜的脂质过氧化损伤具有明显的干预作用,对精子膜结构和功能具有明显的保护作用。

Extract from Morindae officinalis against oxidative injury of function to human sperm membrane

OBJECTIVE: To study the intervention of Morindae officinalis extract in human sperm membrane, and to study the treatment of male infertility and asthenoospermia by M. officinalis. METHOD: To select sperm with normal physiological function using the Percoll gradient centrifugation for the normal sperm model. Then separating the sperm suspension into the normal, model, and control group (Vitamin C group), and the large, medium and small dose of M. officinalis. The ROS was made from hypoxanthine-xanzine xanzine (HX-XO), and ROS, different concentrations (0.125, 0.25, 0.5 mg x mL(-1) of the extract were hatched with sperm in the oxygen environment, the sperm membrane Lipid peroxide injury were analyzed, and the function of sperm membrane were analyzed by sperm Hypoosmoticswelling (HOS) and compared with the controlled group. RESULT: In the same conditions, all the small, medium and large extracts of M. officinalis (0.125, 0.25, 0.5 g x mL(-1)) improved SOD vitality of sperm suspension, reduced the content of MDA, intervened in the injury of sperm membrane by ROS to some extent and protected some function of sperm membrane. The 0.125 mg x mL(-1) extract had no obvious difference (P > 0.05) with Vitamin C in it, but the (0.25, 0.5 mg x mL(-1)) concentration of the extract is significantly better than control Vitamin-C (P < 0.01, P < 0.001). Furthermore, there was a dependence on the dosage, the large dose (0.5 mg x mL(-1)) of M. officinalis especially protected the function of sperm membrane. CONCLUSION: The extract from M. officinalis can significantly intervene in lipin peroxidation in sperm membrane by guarding against oxidation, and protect the structure and function of sperm membrane, that is one of the mechanisms for treating male's infertility and asthenoospermia with M. officinalis.