| 鼻咽癌细胞株中C-KIT表达和突变以及对Imatinib的反应性 |
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| 引用本文： | 黄培钰,洪明晃,张星,麦海强,罗东华,张力.鼻咽癌细胞株中C-KIT表达和突变以及对Imatinib的反应性[J].癌症,2010,29(2):137-142. |
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| 作者姓名： | 黄培钰 洪明晃 张星 麦海强 罗东华 张力 |
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| 作者单位： | 华南肿瘤学国家重点实验室;中山大学肿瘤防治中心鼻咽癌科;中山大学肿瘤防治中心生物治疗中心;中山大学肿瘤防治中心内科; |
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| 基金项目： | 广东省医学科研基金(No.B2007067);;No.985-Ⅱ华南肿瘤学国家重点实验室资助~~ |
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| 摘 要： | 背景与目的:我们先前报道了鼻咽癌组织中存在C-KIT表达和突变,但体外水平Imatinib是否对鼻咽癌细胞有增殖抑制作用及其机制尚不清楚。本研究评价了鼻咽癌细胞株中C-KIT表达和突变以及对Imatinib的反应性,探讨三者之间的相关性。方法:应用Western blot检测鼻咽癌细胞株CNE-1、CNE-2、Hone-1、C-666、SUNE-1、5-8F及鼻咽上皮细胞株NP-69的C-KIT表达情况,应用直接测序法检测CNE-1、CNE-2、Hone-1、C-666、SUNE-1、5-8F、NP-69的C-KIT基因突变情况,应用CCK-8试剂盒进行Imatinib对CNE-1、CNE-2、Hone-1、C-666、SUNE-1及5-8F的增殖抑制实验,应用Pearson相关分析和t检验分析鼻咽癌细胞株中C-KIT表达、突变和对Imatinib的反应性三者之间是否有相关性。结果:鼻咽癌CNE-1、CNE-2、Hone-1、C-666、SUNE-1及5-8F细胞株相对于鼻咽上皮细胞株NP-69存在C-KIT蛋白高表达。CNE-1、CNE-2、Hone-1、NP-69发现杂合IVS17+78T>C,C-666...
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| 关 键 词： | 鼻咽肿瘤 C-KIT 蛋白表达 基因突变 增殖抑制作用 |
C-KIT overexpression and mutation in nasopharyngeal carcinoma cell lines and reactivity of Imatinib on these cell lines |
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| Pei-Yu Huang,Ming-Huang Hong,Xing Zhang,Hai-Qiang Mai,Dong-Hua Luo,Li Zhang,.State Key Laboratory of Oncology in South China,Guangzhou,Guangdong ,P.R.China.C-KIT overexpression and mutation in nasopharyngeal carcinoma cell lines and reactivity of Imatinib on these cell lines[J].Chinese Journal of Cancer,2010,29(2):137-142. |
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| Authors: | Pei-Yu Huang Ming-Huang Hong Xing Zhang Hai-Qiang Mai Dong-Hua Luo Li Zhang State Key Laboratory of Oncology in South China Guangzhou Guangdong PRChina |
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| Institution: | Pei-Yu Huang1,2,Ming-Huang Hong1,Xing Zhang1,3,Hai-Qiang Mai1,Dong-Hua Luo1,Li Zhang1,41.State Key Laboratory of Oncology in South China,Guangzhou,Guangdong 510060,P.R.China 2.Department of Nasopharyngeal Carcinoma,Sun Yat-sen University Cancer Center,P.R.China 3.Department of Biotherapy,P.R.China 4.Department of Medical Oncology,P.R.China |
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| Abstract: | Background and Objective:We previously reported that C-KIT overexpression and mutation exist in biopsy samples of nasopharyngeal carcinoma (NPC).Yet whether Imatinib had an inhibitory effect on the proliferation of NPC in vitro was still unknown.So,this study examined whether sensitivities to Imatinib of other cell lines are different and whether CKIT expression and mutations exist,to analyze the correlations between them.Methods:The expression of C-KIT in NPC cell lines,including CNE-1,CNE-2,Hone-1,C-666,S... |
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| Keywords: | Nasopharyngeal carcinoma C-KIT protein expression gene mutation proliferation inhibitory effect |
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