| 肌动蛋白是大鼠肝细胞核基质的组分之一 |
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| 引用本文： | 李璟,温博贵,吴健谊,陈爱云. 肌动蛋白是大鼠肝细胞核基质的组分之一[J]. 汕头大学医学院学报, 2001, 14(1): 13-14,16 |
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| 作者姓名： | 李璟 温博贵 吴健谊 陈爱云 |
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| 作者单位： | 汕头大学医学院生物化学教研室 |
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| 基金项目： | 中国预防医学科学院立项课题 |
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| 摘 要： | 目的：证实肌动蛋白存在于大鼠肝细胞核基质中。方法：应用SDS－PAGE法分析大鼠肝细胞核基质的组分，以肌动蛋白多克隆抗体进行Westem Blot免疫印迹检测。结果：核基质蛋白SDS－PAGE图谱中可见相当于肌动蛋白的43KD条带，Westem Blot分析证实核基质中存在肌动 蛋白条带。结论：肌动蛋白是大鼠肝细胞核基质的组分之一。
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| 关 键 词： | 肌动蛋白 大鼠 肝细胞 核基质 SDS－PAGE法 |
| 文章编号： | 1007-4716（2001）01-0013-03 |
Actin is a Component ofthe Nuclear Matrices of Rat Hepatocytes |
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| Li Jing,Wen Bogui,Wu Jianyi,Chen Aiyun. Actin is a Component ofthe Nuclear Matrices of Rat Hepatocytes[J]. Journal of Shantou University Medical College, 2001, 14(1): 13-14,16 |
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| Authors: | Li Jing Wen Bogui Wu Jianyi Chen Aiyun |
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| Abstract: | Objective:To determine actin in the nuclear matrices of the hepatocytes of rats.Methods:The nuclear matrices of hepatocytes were analyzed by SDS_PAGE and the actin was detected by Western Blot.Results:The polypeptide of 43 KD was found to occur in the nuclear matrices of the hepatocytes.It was determined to be actin by Western Blot analysis.Conclusion:The actin was demonstrated to be a component of the nuclear matrices of hepatocytes of rats. |
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| Keywords: | Actin Rat Hepatocyte Nuclear Matrix |
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