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Intravital microscopy visualizes innate immune crosstalk and function in tissue microenvironment |
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| Authors: | Jin Wang Dong Dong Wenying Zhao Jing Wang |
| Affiliation: | 1. Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China Jin Wang, Dong Dong, and Wenying Zhao contributed equally to this study.;2. Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Jin Wang, Dong Dong, and Wenying Zhao contributed equally to this study.;3. Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China |
| Abstract: | Significant advances have been made in the field of intravital microscopy (IVM) on myeloid cells due to the growing number of validated fluorescent probes and reporter mice. IVM provides a visualization platform to directly observe cell behavior and deepen our understanding of cellular dynamics, heterogeneity, plasticity, and cell–cell communication in native tissue environments. This review outlines the current studies on the dynamic interaction and function of innate immune cells with a focus on those that are studied with IVM and covers the advances in data analysis with emerging artificial intelligence-based algorithms. Finally, the prospects of IVM on innate immune cells are discussed. |
| Keywords: | Innate immune cells Intravital imaging Microenvironment Deep learning |