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| 宁夏回族5个Y-STR基因座及单倍型遗传多态性分析 | |
| 引用本文： | 朱波峰,张飞雄,刘新社,吕桂平,钱光立,朱军,刘耀.宁夏回族5个Y-STR基因座及单倍型遗传多态性分析[J].第四军医大学学报,2005,26(14):1275-1278. |
| 作者姓名： | 朱波峰 张飞雄 刘新社 吕桂平 钱光立 朱军 刘耀 |
| 作者单位： | 1. 西安交通大学环境与疾病相关基因教育部重点实验室,陕西,西安,710061 2. 宁夏医学院附属医院消化内科,宁夏,银川,750004 3. 山东省公安厅物证鉴定中心,山东,济南,255000 4. 云南省弥勒县公安局物证鉴定中心,云南,弥勒,652300 5. 西安交通大学环境与疾病相关基因教育部重点实验室,陕西,西安,710061;公安部物证鉴定中心,北京,100038 6. 公安部物证鉴定中心,北京,100038 |
| 摘 要： | 目的:研究宁夏回族5个Y-STR基因座及单倍型遗传多态性,估价它们在法医学中的应用价值和丰富中华民族基因信息资源.方法:调查宁夏回族101个无关、健康男性个体.研究5个Y-STR基因座基因及单倍型频率分布.每个基因座的引物分别用FAM,HEX或TAMRA荧光标记,通过复合扩增、基因扫描、基因分型和遗传多态性分析获得数据.结果:5个Y-STR基因座共检测到29个等位基因,频率分布在0.0099～0.5446.101个个体共检测到94个单倍型,87个是唯一的,有7个单倍型被检测2次.单倍型的多样性为0.9986.结论:该数据可以很好的应用于法医学个体识别和亲权鉴定以及群体遗传学研究. |
| 关 键 词： | Y染色体 串联重复序列 多态现象(遗传学) 单倍性 |
| 文章编号： | 1000-2790(2005)14-1275-04 |
| 修稿时间： | 2004年10月14 |
Genetic polymorphisms of 5 Y chromosomal STRs loci and haplotypes in Hui ethnic group |
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| ZHU Bo-Feng,ZHANG Fei-xiong,LIU Xin-she,L Gui-Ping,QIAN Guang-Li,ZHU Jun,LIU Yao.Genetic polymorphisms of 5 Y chromosomal STRs loci and haplotypes in Hui ethnic group[J].Journal of the Fourth Military Medical University,2005,26(14):1275-1278. | |
| Authors: | ZHU Bo-Feng ZHANG Fei-xiong LIU Xin-she L Gui-Ping QIAN Guang-Li ZHU Jun LIU Yao |
| Affiliation: | ZHU Bo-Feng,ZHANG Fei-xiong,LIU Xin-she,L(U) Gui-Ping,QIAN Guang-Li,ZHU Jun,LIU Yao |
| Abstract: | AIM: To study genetic polymorphisms of 5 Y chromosomal STRs in Hui ethnic group living in Ningxia Hui Autonomous Region and to assess their value in forensic science. METHODS: We investigated 101 unrelated, healthy, male individuals of Hui ethnic group and studied their allelic frequency distributions and haplotype diversity of 5 Y chromosomal STRs. Primer for each loci was labeled with the fluorescence, such as FAM (blue), HEX (green) or TAMRA (yellow). The data of Hui ethnic group were obtained through co-amplification, GeneScan, genotyping, and genetic distribution analysis. RESULTS: Twenty-nine alleles were detected, with frequencies ranging from 0.0099 -0.5446. Out of the total of 101 individuals, 94 were of different haplotypes, 87 were unique and 7 were found 2 times. The haplotype diversity for 5 Y-STR loci was 0.9986. CONCLUSIONS: The data can be well useful for individual identification and paternity testing in forensic fields and in the study of population genetics. |
| Keywords: | Y chromosome tandem repeat sequences polymorphism (genetics) haploidy |
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