| 高效液相色谱法测定注射用甲硫氨酸维B1中维生素B1含量 |
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| 引用本文： | 禹学军,钱红君.高效液相色谱法测定注射用甲硫氨酸维B1中维生素B1含量[J].中国药业,2008,17(4):34-35. |
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| 作者姓名： | 禹学军 钱红君 |
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| 作者单位： | 吉林敖东药业集团延吉股份有限公司,吉林,延吉,133001 |
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| 摘 要： | 目的探讨用高效液相色谱（HPLC）法测定注射用甲硫氨酸维B1的维生素B1含量。方法采用C18色谱柱（150mm×4．6mm，5μm），以混合溶液（取庚烷磺酸钠12g，加少量水溶解，再加三乙胺12mL，加水稀释至1000mL，用磷酸调pH值至3．2即可）-甲醇（80：20）为流动相，检测波长为246nm。结果维生素B1质量浓度在4—32μg／mL范围内与峰面积线性关系良好，r=0．9993，平均回收率为100．3％，RSD为0．54。结论HPLC法精密可靠，可作为注射用甲硫氨酸维B1的质量控制方法。
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| 关 键 词： | 甲硫氨酸维B1 维生素B1 高效液相色谱法 |
| 文章编号： | 1006-4931(2008)04-0034-02 |
| 修稿时间： | 2007年2月10日 |
Determination of Vitamin B1 in Methionine and Vitamin B1 for Injection by HPLC |
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| Yu Xuejun,Qian Hongjun.Determination of Vitamin B1 in Methionine and Vitamin B1 for Injection by HPLC[J].China Pharmaceuticals,2008,17(4):34-35. |
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| Authors: | Yu Xuejun Qian Hongjun |
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| Institution: | (Yanji Co. Ltd., Jilin Aodong Pharmaceutical Industry Group, Yanji, Jilin, China 133001) |
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| Abstract: | Objective To estalish a method for the determination of vitamin B1 in methionine and vitamin B1 for injection by HPLC. Methods HPLC conditions were CIS column（150 mm×4. 6 mm, 5μm）, mixtures（extracted sodium heptanesulfonate 12 g, solved with a small amount of water, then added triethylamine 12 mL, diluted to 1 000 mL water, pH was adjusted to 3.2 with phosphoric acid）-methanol（80：20）as the mobile phase and 246 nm as the detective wave length. Results The linear range of vitamin B1 was 4- 32 g/mL, r=0. 999 3. The average recovery rate was 100. 3%, RSD was 0. 54. Conclusion This method is precise and credible. It can be applied to the quality control of methionine and vitamin B1 for injection. |
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| Keywords: | methionine and vitamin B1 vitamin B1 HPLC |
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