荧光定量PCR测定支气管肺泡灌洗液中TbDNA对涂阴肺结核的诊断价值

目的探讨纤支镜肺泡灌洗液中结核分枝杆菌DNA在涂阴肺结核诊断中的价值。方法应用荧光定量聚合酶链反应（FQ-PCR）技术对300例涂阴肺结核，178例菌阳肺结核及119例肺炎或肺癌患者的纤支镜肺泡灌洗液标本进行结核分枝杆菌DNA检测。结果3种病因肺泡灌洗液结核分枝杆菌DNA阳性检出率分别为：74.3%（223/300）、94.9%（169/178）、17.6%（21/119）。结论荧光定量聚合酶链反应检测肺泡灌洗液结核分枝杆菌DNA，用于肺结核诊断可提高肺结核诊断的敏感性和准确性，明显优于痰抗酸染色，亦较痰结核菌培养诊断灵敏、快速，可作为肺结核诊断较可靠指标。

The clinical value of Tb-DNA in BALF detected by FQ-PCR in diagnosing smear-negative pulmonary tuberculosis

Objective To study the value of M. tuberculosis （MTB） DNA（MTB-DNA） in bronchoalveolar lavage fluid （BALF） detected by fluorescence quantitative polymerase chain reaction （FQ-PCR） in diagnosing smear-negative pulmonary tuberculosis. Methods FQ-PCR was uesd to detect the MTB-DNA in BALF from 300 smear-negative pulmonary TB patients, 178 smear-positive pulmonary TB patients and 119 patients with pneumonia or lung cancer. Results The positive rates of MTB-DNA in 3 groups patients mentioned above were 74. 3%（223/300）, 94.9%（169/178）, and 17.6%（21/119）, respectively. Conclusion Detecting MTB-DNA in BALF by FQ-PCR could significantly improve the sensitivity of the diagnosis of TB, might become a helpful method for the diagnosis of TB.