人参皂甙改善老龄小鼠学习记忆的研究

目的探讨人参皂甙对自然衰老的C57BL/6J小鼠学习记忆的改善作用及其机制。方法20月龄的C57BL/6J小鼠80只,随机分为老年对照组和25、50、100mg/(kgbw·d)人参皂甙干预组,另设3月龄青龄对照组(n=20)。人参皂甙随饮水摄入,共干预6个月。干预结束后应用Morris水迷宫试验检测小鼠的空间学习记忆能力;并检测各组小鼠血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及MDA含量;Nissl染色观察海马神经元数量的改变;Westernblot方法观察海马区脑源性神经营养因子(BDNF)的表达。结果50,100mg/(kgbw·d)人参皂甙干预6个月后能明显提高小鼠的空间学习记忆能力;血清中SOD、GSH-Px活性明显增强,MDA含量显著下降;海马BDNF蛋白的表达明显增强。25mg/(kgbw·d)人参皂甙干预后血清GSH-Px的活性显著提高。各组动物海马神经元数量无明显差别。结论50、100mg/(kgbw·d)人参皂甙干预能够延缓老龄小鼠学习记忆功能的减退,减轻机体氧化损伤,增强海马区BDNF蛋白的表达可能是其重要的作用机制。

GINSENOSIDE FACILITATES LEARNING AND MEMORY IN AGED MICE

Objective To investigate the effect of ginsenoside on degeneration of learning and memory in aged mice and the mechanism.Method Eighty female C57BL/6J mice aged 20-mo were randomly divided into drinking.In addition,20 female C57BL/6J mice aged 3 mo were used to be young control group.Eight mo later,the learning and memory abilities of the mice were checked by Morris water maze.Thereafter,the mice were killed by decapitation,and their serum was collected to determine the level of SOD,GSH-Px and MDA.Hippocampal morphology was examined by Nissl stain;and the expression of brain-derived neurotrophic factor administration could significantly shorten the escape latency of the aged mice in Morris water maze.Furthermore,the alleviated oxidative stress and up-regulated expression of BDNF were observed in 50,delay the degeneration of learning and memory in aged mice by lowering oxidative damage and up-regulating BDNF expression in hippocampus.