早期给予膳食三羟异黄酮对大鼠母代生殖及其子代生长发育的影响

目的研究早期给予三羟异黄酮(Gen)对母鼠生殖及其雌性子代发育的影响。方法母鼠孕10d时,随机分为3组,每组6只,饲以含不同剂量Gen的基础饲料(对照组0mg/kg/d,中剂量组20mg/kg/d,高剂量组100mg/kg/d)。每周检测母鼠和仔鼠生长指标,产后D21处死母鼠和8只雌性仔鼠,取血,分离雌性仔鼠子宫、卵巢,进行雌二醇测定与病理检查,用Western blotting测定子宫、卵巢中雌激素受体(ER)的表达水平;后将各剂量组母鼠所产仔鼠分别抽取体重相似的雌性仔鼠各24只,随机分为3组,每组各8只,待其发育至13w龄时全部处死,处理方法同上。结果与对照组相比,高剂量组21d时死亡率高于对照组,幼鼠子宫、卵巢称重减少,病理切片发现高剂量组雌幼鼠卵泡发育提前,雌性仔鼠体重9w龄左右开始下降,阴道开放时间提前;13w龄时,血清雌二醇水平降低,病理切片示子宫腔扩大,子宫扩张,子宫、卵巢雌激素受体ER表达量降低。结论早期给予Gen对大鼠的生长发育影响较大,而且对大鼠体重的影响主要在于其成年后。这种影响与其对激素水平和受体表达有关。

EFFECT OF EARLY ADMINISTRATION OF DIETARY GENISTEIN ON REPRODUCTIVE DEVELOPMENT IN TWO GENERATIONAL RATS

Objective To investigate the effects of early administration of dietary genistein(Gen) on the reproductive development of two generational rats. Method Rats were divided into 3 groups randomly on their pregnant day 10. They were fed with basic diet containing different dose of Gen (control:0 mg/kg·d,middle Gen:20mg/kg·d,high Gen:100 mg/kg·d). Growth indices were detected every week,At age of 21 d,all mother rats and 8 female pups were killed and their blood,uterus and ovaries were taken for estradiol determination and pathological examination. Western blotting was used to measure the expression of estradiol receptor (ER) in uterus and ovary. After that,8 female pups of similar body weights from every group were killed at age of 13w and treated similarly. Results Compared with control group,pup mortality at 21d old was increased in high Gen group,and uterus and ovary weight in female rats were deceased. Histopathology showed that more vesicula graafianae appeared in ovary of 3w old female pups in high Gen group. At 9w old,the body weight of female rats decreased,and the vaginal open time was earlier than control group. At age of 13w,the plasma estradiol level decreased. The cavity of uterus was larger than control group. Treatment with genistein augmented the expression of ER in uterus and ovary. Conclusion Genistein at early life period can influence the reproductive development of female rats. The effect on rats,body weight was mainly after growing up. It seems that the change of hormone level and expression of ER is related with these effects.