A simple method for purification of genomic DNA from whole blood using Fe3O4/Au composite particles as a carrier |
| |
| Authors: | Zhao Ming Zhang Xianqing Wang Sen Chen Chao Cui Yali |
| |
| Institution: | 1. College of Life Sciences, Northwest University, Xi''an 710069, China;2. Department of Blood Transfusion, Xijing Hospital, Fourth Military Medical University, Xi''an 710032, China;1. Centre for Water Management and Reuse, School of Natural and Built Environments, University of South Australia, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia;2. State Key Laboratory for Environmental Aquatic Chemistry, Chinese Academy of Sciences, Beijing, China;3. Australian Water Quality Centre, 250 Victoria Square, Adelaide, SA 5000, Australia;4. Barbara Hardy Institute, University of South Australia, Australia;1. Institute of Brain Science, National Yang Ming University, Taipei, Taiwan;2. Sleep Research Center, National Yang Ming University, Taipei, Taiwan;3. Brain Research Center, National Yang Ming University, Taipei, Taiwan;4. Department of Education and Research, Taipei City Hospital, Taipei, Taiwan;5. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan |
| |
| Abstract: | ObjectiveTo establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier.MethodsTwo crucial conditions (sodium chloride concentration and amount of the magnetic particles) were optimized and 8 different human whole blood samples were used to purify genomic DNA under the optimal condition. Then agarose gel electrophoresis and polymerase chain reaction (PCR) were performed.ResultsThe optimal binding condition was 1.5 mol/L NaCl/10% PEG, and the optimal amount of Fe3O4/Au composite particles was 600 μg. The yields of the genomic DNA from 100 μl of different whole blood samples were 2-5 μg, and the ratio of A260/A280 was in the range of 1.70-1.90. The size of genomic DNA was about 23 kb and the PCR was valid.ConclusionThe purification system using Fe3O4/Au composite microparticles has advantages in high yield, high purity, ease of operating, time saving and avoiding centrifugation. The purified sample was found to function satisfactorily in PCR amplification. |
| |
| Keywords: | Fe3O4/Au composite particles Genomic DNA Purification Whole blood |
| 本文献已被 维普 万方数据 ScienceDirect 等数据库收录！ |
|
