雌激素受体基因XbaI多态性与补钙对青春期少女骨骼发育作用的关系

目的研究雌激素受体(ER)基因XbaI多态性与补钙对青春期少女骨骼发育作用的关系,为制定钙膳食参考摄入量提供科学依据。方法从志愿者中选取88名13~15岁青春期少女,随机分为补钙组和对照组,并按XbaI多态性分成不同的亚组,进行为期1年的补钙实验。在补钙前后测定全身各部位骨密度、骨钙素(BGP)、骨碱性磷酸酶(BAP)、抗酒石酸酸性磷酸酶(TRACP)、雌二醇(E2)及维生素D水平。比较不同基因亚型间补钙后骨密度增长或骨生化指标变化的差异。结果共72人完成了整个研究。经过1年的干预,补钙组BGP的增长及前臂远端1/10处的骨密度增长均大于对照组;补钙组内的Xx亚型反映骨形成的指标BAP的增长大于xx亚型,且全身各测量部位的骨密度增长值均大于xx亚型,但无显著性差异;而对照组内各XbaI亚型上述各指标的增长值未见统计学差异。结论XbaI多态性中Xx较xx亚型具有更好的补钙反应性,在今后的补钙干预中应考虑该遗传因素的影响。

ASSOCIATION OF ESTROGEN RECEPTOR-ALPHA GENE Xbal POLYMORPHISMS AND THE EFFECT OF CALCIUM SUPPLEMENTATION ON SKELETAL DEVELOPMENT IN CHINESE PUBERTAL GIRLS

Objective To investigate the association of estrogen receptor-αXbaI polymorphisms concerned with the effect of calcium supplementation on bone mineral density(BMD) and bone turnover markers of Chinese pubertal girls.Method Eighty-eight pubertal girls aged 13-15 years were selected and divided into 2 groups: control and supplemented group.Each group included two subgroups according to the ER-αXbaI polymorphism.BMD,bone turnover markers(BGP,BAP,TRACP),25-OH-VitD3,E2 were measured at baseline and 12 months after calicum supplementation.Results Seventy-two girls completed 12-month study period.The absolute increase of BGP and BMD in distal 1/10 forearm of supplemented group was significantly higher than that in the control group.In the supplemented group,the increase of BAP in Xx genotype was higher than xx genotype and all detected sites BMD in Xx genotype were higher than xx,but were not statistically different.No difference was found in all above index between the two XbaI geontypes in the control group.Conclusion Xx genotype showed better response to calcium supple-mentation than xx,and this hereditory factor should be considered in calcium supplementation.