花色苷抗氧化及改善胰岛素抗性的体外研究

目的研究花色苷(Anth)体外抗氧化及改善胰岛素抗性(IR)的效应,并分析构效关系。方法以自然界最为常见的6种Anth作为受试物,测定其对二苯代苦味酰肼(1-diphenyl-2-picrylhydrazyl,DPPH?)自由基的清除能力,及其对H2O2诱导的IR3T3-L1脂肪细胞胞内活性氧(reactive oxygen species,ROS)水平和葡萄糖摄取能力的影响。结果6种Anth均具有较强的DPPH自由基清除能力,效应和B环上酚羟基数目有关;6种Anth中,具有邻苯二酚结构的2种能更好的清除H2O2诱导IR脂肪细胞胞内的ROS,同时显著提高胰岛素刺激后脂肪细胞对葡萄糖的摄取能力,呈剂量-效应依赖型。结论花色苷可预防和改善氧化应激引起的3T3-L1脂肪细胞胰岛素抗性,此效应与B环上的邻苯二酚结构有关。

STRUCTURE-ACTIVITY RELATIONSHIPS OF ANTHOCYANINS IN PROTECTION AGAINST OXIDATIVE STRESS-INDUCED INSULIN RESISTANCE IN VITRO

Objective To explore the structure-activity relationships of anthocyanins (Anth) in protection against oxidative stress-induced insulin resistance (IR) in vitro. Method Six kinds of Anth representing the aglycons, were used to evaluate their free radical scavenging activity by DPPH method, and then the influence on intracellular ROS level and insulin-stimulated glucose uptake were determined in 3T3-L1 adipocytes treated with micromolar hydrogen peroxide for 12 h. Results All six kinds of Anth could rapidly scavenge DPPH free radical, of which only two with ortho-dihydroxyphenyl structure on the B-ring suppressed the intracellular ROS generation and improved insulin-stimulated glucose uptake in adipocytes in dose- dependent manner. Conclusion Anthocyanins could protect 3T3-L1 adipocytes against oxidative stress- induced IR, and the ortho-dihydroxyphenyl structure may contribute to the protection.