乳腺癌组织中uPA、uPAR及nm23-H1的表达

目的　观察乳腺癌组织中uPA、uPAR、nm2 3 H1的表达并探讨与腋窝淋巴结转移的关系。方法　用免疫组化EnVi sion两步法检测 6 9例乳腺癌组织中uPA、uPAR和nm2 3 H1表达的分布情况 ,观察其与肿瘤的分化程度以及与腋窝淋巴结转移的关系。结果　 (1)uPA阳性表达定位于癌细胞胞质 ;uPAR和nm2 3 H1阳性表达定位于癌细胞胞膜及胞质 ,多数癌旁乳腺上皮细胞呈nm2 3 H1阳性表达 ;高分化乳腺癌 (Ⅰ级 )uPA和uPAR表达阳性率 (30 0 %和 2 5 0 %)低于中低分化乳腺癌 (Ⅱ、Ⅲ级 ) (分别为 6 8 1%、72 7%和 70 0 %、74 1%) (P <0 0 5 ) ;nm2 3 H1表达阳性率在乳腺癌组织不同分化程度间差异无显著性 (P >0 0 5 ) ;(2 )腋窝淋巴结有转移者uPA和uPAR的表达阳性率 (73 2 %和 75 6 %)高于无淋巴结转移者 (35 7%和35 7%) (P <0 0 5 ) ;有腋窝淋巴结转移者nm2 3 H1的表达阳性率 (2 4 4 %)显著低于无淋巴结转移者 (5 0 0 %) (P <0 0 5 ) ;uPA、uPAR和nm2 3 H1的表达与淋巴结转移的个数均无关 ;(3)uPA阳性表达的癌组织其nm2 3 H1表达阳性率 (15 0 %)低于uPA阴性表达的癌组织 (6 2 1%) (P <0 0 5 )。结论　uPA和uPAR的高表达与乳腺癌腋窝淋巴结转移密切相关 ;uPA、uPAR和nm2 3 H1可以作为乳腺癌侵袭与淋巴结转移的

Expression of uPA,uPAR and nm23-H1 gene in human breast carcinoma

Purpose To study the expression of urokinase-type plasminogen (uPA)?its receptor(uPAR) and nm23-H1 gene in human breast carcinoma and its relationship to axillary lymph node metastasis. Methods The expression of uPA, uPAR and nm23-H1 gene in 69 cases of human breast carcinoma was tested by EnVision two-step immunohistochemical method. Results uPA expression was located in the cytoplasm of breast carcinoma cells while uPAR and nm23-H1 gene expression was located in the membrane and cytoplasm of breast carcinoma cells. The positivity rate of uPA (30.0%) and uPAR (25.0%) in high differential carcinoma were significantly lower than those in intermediate and low differential carcinoma(68.1%,72.7% and 70.0%,74.1% respectively)(P<0.05). And the positivity rate of uPA (73.2%) and uPAR (75.6%) in carcinomas with axillary lymph node metastasis were significantly higher than those without axillary lymph node metastasis (35.7% and 35.7%)(P<0.05). On the contrary, the positivity rate of nm23-H1 gene expression (24.4%) in carcinomas with axillary lymph node metastasis was significantly lower than those without axillary lymph node metastasis (50.0%)(P<0.05). And the positivity rate of nm23-H1 gene expression (15.0%) in carcinoma with uPA positively expressed was significantly lower than those in carcinoma with uPA negatively expressed(62.1%)(P<0.05). Conclusions uPA?uPAR and nm23-H1 gene may be an important biological marker in invasion and lymph node metastasis of breast carcinoma.