| 重组人白细胞介素-13对巨核细胞系-Dami细胞原癌基因c-mpl表达的影响 |
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| 引用本文： | 李克强,李文林,石小玉,熊丽霞.重组人白细胞介素-13对巨核细胞系-Dami细胞原癌基因c-mpl表达的影响[J].江西医学院学报,2001,41(5):1-3. |
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| 作者姓名： | 李克强 李文林 石小玉 熊丽霞 |
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| 作者单位： | 江西医学院病理生理学教研室 江西南昌330006
(李克强，李文林)，江西医学院形态学中心实验室 江西南昌330006
(石小玉)，江西医学院病理生理学教研室 江西南昌330006(熊丽霞) |
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| 基金项目： | 国家自然科学基金资助 (NO .3 9860 0 3 3 )，江西省自然科学基金资助 (NO .984 0 17) |
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| 摘 要： | 目的:研究重组人白细胞介素-13(rhIL-13)对巨核细胞系-Dami细胞原癌基因c-mpl表达的影响.方法:实验组细胞培养系统中分别加入25 ng/ml,50 ng/ml,100ng/ml rhIL-13,对照组不加任何细胞因子.用RT-PCR法检测c-mpl mRNA的表达.结果:经25 ng/ml、50 ng/ml、100ng/ml rhIL-13处理过的实验组Dami细胞较之对照组其c-mpl mRNA的表达分别提高24.8%、27.9%和31.5%.结论:rhIL-13增强了Dami细胞原癌基因c-mpl表达;rhIL-13促进Dami细胞增殖的部分机制可能是通过上调c-mpl的表达来实现的.
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| 关 键 词： | 重组人白细胞介素-13 Dami细胞 c-mpl |
| 文章编号： | 1000-2294(2001)05-0001-03 |
| 修稿时间： | 2001年7月17日 |
The Effect of Recombinant Human Interleukin-13 on Expression of Proto-Oncogene c-mpl of Dami Cells |
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| LI Ke qiang ,LI Wen lin ,SHI Xiao yu ,XIONG Li xia.The Effect of Recombinant Human Interleukin-13 on Expression of Proto-Oncogene c-mpl of Dami Cells[J].Acta Academiae Medicinae Jiangxi,2001,41(5):1-3. |
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| Authors: | LI Ke qiang LI Wen lin SHI Xiao yu XIONG Li xia |
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| Institution: | LI Ke qiang 1,LI Wen lin 1,SHI Xiao yu 2,XIONG Li xia 1 |
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| Abstract: | Objective: To investigate the effect of recombinant human interleukin 13(rhIL 13) on the expression of proto oncogene c mpl in Dami Cells, a human megakaryobiastic leukemia cell line. Methods:In experimental groups,the concentrations of rhIL 13 were 25 ng/ml, 50 ng/ml,and 100ng/ml. In control group, the concentration of rhIL 13 was 0 ng/ml. The expression of c mpl mRNA in Dami Cells was determined with RT PCR. Results:The expressions of c mpl mRNAs in groups of 25 ng/ml, 50 ng/ml, and 100ng/ml increased by 24.8%?27.9%, and 31.5%, respectively. Conclusion:The data indicate that rhIL 13 enhances the expression of proto oncogene c mpl in Dami Cells. Moreover, the stimulation of Dami Cells by rhIL 13 may be partly resulted from an increase of the proto oncogene c mpl gene expression. |
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| Keywords: | recombinant human interlukin 13(rhIL 13) Dami cell c mpl |
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