| 山茱萸多糖的分离纯化及部分理化性质研究 |
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| 引用本文： | 舒晓燕,胡定慧,侯大斌,阮期平.山茱萸多糖的分离纯化及部分理化性质研究[J].中国现代中药,2006,8(7):15-17. |
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| 作者姓名： | 舒晓燕 胡定慧 侯大斌 阮期平 |
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| 作者单位： | 1. 西南科技大学生命科学与工程学院,四川,绵阳,621000
2. 绵阳师范学院分子生物学与生物制药重点实验室,四川,绵阳,621000 |
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| 基金项目： | 绵阳师范学院校科研和教改项目 |
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| 摘 要： | 目的:从山茱萸果实中分离出山茱萸多糖,并研究其理化性质。方法:新鲜山茱萸果实经热水浸泡后在碱性条件下提取、脱蛋白、透析、乙醇沉淀、DEAE-cellulose柱色谱分离,再经过SephacrylS-300凝胶柱色谱进一步纯化。结果:得到一种白色粉末状均一酸性多糖M。结论:多糖M包含2个单糖。
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| 关 键 词： | 山茱萸 多糖 分离纯化 理化性质 |
| 收稿时间： | 05 11 2006 12:00AM |
| 修稿时间： | 2006年5月11日 |
Study on Purification and Physicochemical Properties of Polysaccharide from Cornus officinalis |
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| Shu Xiaoyan,Hu Dinghui,Hou Dabin and Ruan Qiping.Study on Purification and Physicochemical Properties of Polysaccharide from Cornus officinalis[J].Modern Chinese Medicine,2006,8(7):15-17. |
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| Authors: | Shu Xiaoyan Hu Dinghui Hou Dabin and Ruan Qiping |
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| Institution: | 1. College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621000, China; 2. Key Laboratory for Molecular Biology and Biopharmaceuticals, Mianyang Normal University, Mianyang, Sichuan 621000, China |
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| Abstract: | Objective:To study the isolation and physicochemical properties of polysaccharide from Cornus officinalis. Methods:Hot-water extraction,ethanol precipitation,DEAE-cellulose column chromatography and Sephacryl S-300 gel chromatography were used to isolate and purify the polysaccharide in Cornus officinalis.Results:A homogenous polysaccharide M was isolated from fruits of Cornus officinalis. Conclusion:Polysaccharide M contains two monosaccharides. |
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| Keywords: | Cornus officinalis polysaccharide purification physicochemical properties |
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