| 补骨脂酊中补骨脂素、异补骨脂素的含量测定 |
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| 引用本文： | 王霞,靳凤云,张永东.补骨脂酊中补骨脂素、异补骨脂素的含量测定[J].中南药学,2009,7(4):269-271. |
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| 作者姓名： | 王霞 靳凤云 张永东 |
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| 作者单位： | 1. 郴州市第一人民医院,湖南,郴州,423000
2. 贵阳中医学院,贵阳,550002 |
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| 摘 要： | 目的建立补骨脂酊中补骨脂素、异补骨脂素的含量测定方法。方法采用高效液相色谱法，色谱柱为Agilent Eclipse XDB-C18柱（150mm×4．6mm，5μm）．流动相为甲醇-水（40：60）．流速为1．0mL·min^-1；检测波长为246nm。结果补骨脂素在0．022～0．44μg（r=0．9999），异补骨脂素在0．02388～0．4776μg（r=0．9999）具有良好线性关系。补骨脂素平均回收率为98．10％（n=9．RSD=1．20%），异补骨脂素平均回收率为97．74％（n=9，RSD=1．10％）。结论本方法简便、准确、可靠，可用于补骨脂酊中补骨脂素、异补骨脂素的含量测定。
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| 关 键 词： | 补骨脂酊 补骨脂素 异补骨脂素 高效液相色谱法 |
Content determination of psoralen and isopsoralen in Buguzhiding |
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| WANG Xia,JIN Feng-yun,ZHANG Yong-dong.Content determination of psoralen and isopsoralen in Buguzhiding[J].Central South Pharmacy,2009,7(4):269-271. |
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| Authors: | WANG Xia JIN Feng-yun ZHANG Yong-dong |
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| Institution: | 1. First People＇s Hospital of Chenzhou, Chenzhou Hunan 423000; 2. Guiyang College of Traditional Chinese Medicine, Guiyang 550002) |
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| Abstract: | Objective To establish a method to determine the content of psoralen and isopsoralen in Buguzhiding. Methods HPLC method was used with an Agilent Eclipse XDB-C18 column. The mobile phase consisted of methanolwater （40 ： 60）, the flow rate was 1.0 mL· min 1, and the detection wavelength was 246 nm. Results The linear range of psoralen was 0. 022～0. 44 μg （r=0. 999 9）, and the linear range of isopsoralen was 0. 023 88～0. 477 6μg （r=0. 999 9）. The average recovery of psoralen was 98.10% （n=9, RSD=1. 20%）. The average recovery of isop soralen was 97.74% （n=9, RSD=1. 10%）. Conclusion This method is simple, accurate, and reliable. It can be used for the determination of psoralen and isopsoralen in Buguzhiding. |
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| Keywords: | Buguzhiding psoralen isopsoralen HPLC |
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