| 耐碳青酶烯类鲍氏不动杆菌blaOXA基因型检测及同源性分析 |
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| 引用本文： | 李春,沈继录,方亚平,王中新. 耐碳青酶烯类鲍氏不动杆菌blaOXA基因型检测及同源性分析[J]. 中华医院感染学杂志, 2010, 20(7) |
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| 作者姓名： | 李春 沈继录 方亚平 王中新 |
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| 作者单位： | 1. 安徽医科大学第一附属医院检验科,安徽,合肥,230022
2. 安徽医科大学第二附属医院检验科,安徽,合肥,230032 |
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| 摘 要： | 目的了解碳青酶烯类耐药鲍氏不动杆菌的耐药性、blaOXA基因型及同源性。方法琼脂稀释法测定鲍氏不动杆菌对14种抗菌药物的最低抑菌浓度(MIC),PCR扩增ISAba1、blaOXA23和bla OXA-66/OXA-51基因,脉冲场凝胶电泳(PFGE)分析基因同源性。结果20株鲍氏不动杆菌对亚胺培南和美罗培南均耐药,其中19株为多药耐药株,1株ISAba1和blaOXA-23阳性,2株blaOXA-66阳性,16株ISAba1、blaOXA-23和blaOXA-66均阳性,1株以上3种基因全部阴性;PFGE分析结果显示,A型(12株)、B型(2株)、C型(2株)、D型(3株)、E型(1株)。结论医院感染的鲍氏不动杆菌对碳青酶烯类耐药可能与产OXA-23酶有关,并存在院内的克隆传播。
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| 关 键 词： | 鲍氏不动杆菌 碳青酶烯类 脉冲场凝胶电泳 同源性 |
blaOXA Genotype and Genetic Homology of Carbapenem-resistant Acinetobacter baumannii Isolates |
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| LI Chun,SHEN Ji-lu,FANG Ya-ping,WANG Zhong-xin. blaOXA Genotype and Genetic Homology of Carbapenem-resistant Acinetobacter baumannii Isolates[J]. Chinese Journal of Nosocomiology, 2010, 20(7) |
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| Authors: | LI Chun SHEN Ji-lu FANG Ya-ping WANG Zhong-xin |
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| Affiliation: | LI Chun,SHEN Ji-lu,FANG Ya-ping,WANG Zhong-xin (The First Affliated Hospital,Anhui Medical Uiversity,Hefei,Anhui 230022,China) |
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| Abstract: | OBJECTIVE To investigate the resistance,typing of blaOXA and genetic homology of carbapenem-resistant Acinetobacter baumannii.METHODS The minimum inhibitory concentrations(MIC)of fourteen antibiotics were determined by agar dilution method,and ISAba1,blaOXA23 and bla OXA-66/OXA-51 were assayed by PCR.The genetic homology of the isolates was determined by pulsed field gel electrophoresis(PFGE).RESULTS Twenty strains were all resistant to IMP and MEM and 19 of them were multidrug-resistant strains.The number ... |
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| Keywords: | Acinetobacter baumannii Carbapenem PFGE Homology |
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