P53、P2lras蛋白和P—糖蛋白在胃癌中过表达的意义

目的研究胃癌组织p53、p21ras蛋白和P-耐药糖蛋白(P-gp)在胃癌中的表达及临床意义.方法采用免疫组化SABC法检测7例正常胃粘膜、12例慢性萎缩性胃炎、8例粘膜慢性胃炎伴肠型不典型增生和93例胃腺癌组织中p53、p21ras蛋白和P-gp的表达.结果p53、p21ras蛋白和P-gp在正常胃粘膜组、癌前病变组和胃腺癌组中的阳性率分别为0,14.3%,0;20.0%,25.0%,5.0%;55.9%,51.6%,44.1%.p53蛋白、P-gp表达均与淋巴结转移有关(P＜0.05);p21ras蛋白表达与肿瘤的浸润程度有关(P＜0.05);P-gP表达与p53和p21ras表达有显著的协同性(P＜0.05).结论p53和p21ras蛋白常并存于胃癌组织中.p53突变蛋白和P-gp过表达在胃癌淋巴结转移中起重要作用,因此早期进行p53蛋白和P-gp检测有重要的临床意义.

Clinical significance of over-expression of p53,p21ras protein and P-glycoprotein in gastric carcinoma

Objectives:To study the levels of p53,p21 {ras} protein and P glycoprotein(P gp) in tissues of gastric carcinoma tissues and its clinical significance.Methods:20 cases of gastric precancerous lesions and 93 adenocarcinomas and 7 normal control tissues were analyzed for p53,p21 {ras} and P gp by immunohistochemical staining.Results:The positive expressions of p53,p21 {ras} and P gp in normal,precancerous and adenocarcinomas were 0,14 3%,0;20 0%,25 0%,5 0%;and 55 9%,51 6%,44 1%,respectively.A significant correlation was found that both p53 and P gp overexpression were related to lymph node metastasis(P<0 05).p21 {ras} protein was also correlated to invasiveness(P<0 05),Conclusions:There are commonly co expression of p53 mutational protein,p21 {ras} protein and P gp in the tissues of gastric carcinoma.p53 and P gp overexpression may play an important role in the lymph node metastasis of gastric cancer,so that p53 and P gp gene products immunostaining assay at the early stage of gastric cancer may be of great clinical significance.