统计学建模在中药谱-效关系研究中的应用

目的构建钩藤药材中钩藤碱、异钩藤碱、柯诺辛及柯诺辛B四种生物碱对谷氨酸酯诱导PCI2细胞氧化应激抑制作用的谱-效关系模型。方法在获得钩藤药材中钩藤碱、异钩藤碱、柯诺辛及柯诺辛B四种生物碱对谷氨酸酯诱导的PCI2氧化应激的抑制作用的谱-效关系基础上，利用SPSS15．0软件进行分析，建立谱-效关系模型。结果钩藤碱和柯诺辛对PCI2细胞氧化应激有显著的抑制作用，其谱-效方程为：Y=44．301-＋0．255X＋0．356X，，经7批药材验证，谱-效关系式计算值与实际值的偏差率不超过10％。结论该谱-效关系模式可用于预测钩藤对谷氨酸酯诱导的PCI2细胞氧化应激的抑制作用。

Study on application of statistical modeling on chromatogrmn-effect relation in Traditional Chinese Medicine

Objective To establish chromatogram-effect model based on inhibitory effect of four alkaloids （rhynchophyline, isorhynchophylline, corynoxine, corynoxine B ） on oxidative stress of glutamate-induced PC12 cells. Methods Based on the chromatogram-effect relation of inhibitory effect of four alkaloids on the oxidative stress of glutamate-induced PC12 cells, SPSS 15.0 was used to analyse data. Results Oxidative stress of PC12 cells was significantly inhibited by rhynchophyline and corynoxine. The spectrum efficient equation was, Y = 44. 301 ＋ 0.255X1 ＋ 0. 356X3 （ P 〈 0.05 ）. The deviation of predictive value and true value was no more than 10% by data validation from seven samples. Conchlsions The chromatogram-effect model can be used to predict the effect of uncaria macrophylla wall against the oxidative stress of glutamate-induced PC12 cells.