Y-盒结合蛋白1在乳腺癌组织中的表达及临床意义

目的：检测Y-盒结合蛋白1（YB-1）在乳腺癌及癌旁正常乳腺组织中的表达，探讨其与乳腺癌发生、发展的关系。方法收集河北大学附属医院2012年9月至11月期间手术切除的乳腺癌组织（均为术后石蜡病理确诊为浸润性导管癌）标本30例，癌旁正常乳腺组织标本10例。应用免疫组织化学染色的方法检测组织中 YB-1的表达。结果免疫组化结果显示，YB-1在癌旁正常乳腺组织细胞中无表达或低表达；在乳腺癌组织中高表达，阳性表达率为80%，与癌旁正常乳腺组织相比差异有统计学意义（P＜0.05）。YB-1在Ⅰ期乳腺癌组织中的免疫组化评分显著低于Ⅱ期乳腺癌组织（2.6±1.511 vs.4.2±1.182， P＜0.05）。YB-1在无腋窝淋巴结转移的乳腺癌组织中的表达显著低于伴腋窝淋巴结转移的乳腺癌组织（2.8±1.15 vs.5.1±0.74，P＜0.05）。结论 YB-1乳腺癌中表达上调与乳腺癌临床分期及淋巴结转移有关。

Expression of Y box-binding protein 1 and clinical significance in breast cancer

Objective To investigate the expression level of Y box-binding protein 1 （YB-1） and its clinical significance in breast cancer. Methods Thirty cases of surgical removal of breast cancer tissue （diagnosed with invasive ductal carcinoma by postoperative paraffin pathology） and 10 cases of normal breast tissue adjacent to carcinoma were collected during September 2012 to November 2012 in Hebei University Affiliated Hospital. Immunohistochemical staining was performed to detect YB-1 protein levels in breast cancer or adjacent normal breast tissues. Results Immunohistochemical results showed YB-1 protein levels in breast cancer （positive expression rate=80%） were significantly higher than that in adjacent normal breast tissues （P〈0.05）. Immunohistochemical scores of YB-1 in stage I breast cancer were significantly lower than that in stage Ⅱ（2.6 ± 1.511 vs. 4.2 ± 1.182, P〈0.05）. Moreover, YB-1 expression level in breast cancer samples without lymph node metastasis were significant lower than those of breast cancer with lymph node metastasis （2.8 ± 1.15 vs. 5.1 ± 0.74, P〈0.05）. Conclusion YB-1 expression level increases in breast cancer, which is associated with clinical stages and lymph node metastasis of breast cancer.