胰高糖素样肽1在血管再生中的作用及其机制研究

目的：探讨胰高糖素样肽1（GLP-1）对高糖培养的人脐静脉内皮细胞增殖的影响及其可能机制。方法人脐静脉内皮细胞分组培养，正常葡萄糖组（NG，葡萄糖浓度5.5 mmol/L），高葡萄糖组（HG，葡萄糖浓度33 mmol/L），高糖加GLP-1干预组（分为低剂量组，中剂量和高剂量组，浓度分别为1、10、20 nmol/L，分别记为C1、C2、C3组），于培养24 h，48 h和72 h用MTT法检测细胞增殖情况，并用ELISA法检测培养基上清液中血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）的浓度。结果48 h后高糖组细胞增殖明显低于正常对照组，GLP-1干预后能明显促进细胞增殖，以中剂量最为明显（0.6±0.11 vs.0.47±0.09，P＜0.05），72 h这种情况更为明显；高糖培养48 h细胞分泌VEGF明显减少（101.72±6.82） ng/ml vs.（184.66±14.06 ng/ml），P＜0.05]，GLP-1干预后VEGF水平明显增加（P＜0.05），尤其是中剂量组，72 h结果相似。结论 GLP-1能促进细胞分泌VEGF从而改善高糖导致的内皮细胞增殖能力下降。

Effect and mechanism study of GLP-1 in the revascularization

Objective To study the effect and possible mechanism of Glucagon-like peptide-1 （GLP-1） for human umbilical vein endothelial cells （HUVECs） generation in high glucose nutrient medium. Methods HUVECs cultured in groups： normal glucose group（NG, glucose level was 5.5 mmol/L）, high glucose group（HG, glucose level was 33 mmol/L）, high glucose combined with GLP-1groups（low dose group, medium dose group and high dose group, GLP-1 level were 1 nmol/L, 10 nmol/L and 20 nmol/L respectively and marked group C1, group C2 and group C3）, cell multiplication of groups were detected at 24 hours, 48 hours and 72 hours via MTT method and VEGF levels of supernatant were detected with ELISA kit at the same time. Results The cell multiplication of high glucose group was significantly declined than that of the normal group and increased by GLP-1, especially medium dose group （0.6±0.11 vs. 0.47±0.09, P〈0.05）. This phenomenon was more notable at 72 hours. When cells were cultured with high glucose medium for 48 hours VEGF level was significantly decreased than the control group, （101.72±6.82）ng/ml vs. （184.66±14.06）ng/ml, P〈0.05, and increased significantly by GLP-1（P〈0.05）, the medium dose group was more notable, and the results of 72 hours were similar to the 48 hours.Conclusion GLP-1 can improve the multiplication of endothelial cells declined by high glucose through promoting VEGF secretion.